百里香酚对山羊子宫内膜上皮细胞的体外毒活性研究  

The Cytotoxic Effect of Thymol on Goat Endometrial Epithelial Cells

作　　者：闫宝琪 张世栋[1] 董书伟[1] 王东升[1] 那立冬 桑梦琪 杨洪早 严作廷[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,甘肃省新兽药工程重点实验室,兰州730050 [2]甘肃农业大学动物医学院,兰州730070

出　　处：《中国兽药杂志》2017年第4期35-41,共7页Chinese Journal of Veterinary Drug

基　　金：“十二五”国家科技支撑计划重点课题(2012BAD12B03);中国农业科学院科技创新工程项目(CAAS-ASTIP-2014-LIHPS-03);甘肃省自然科学基(1506RJYA146)

摘　　要：为了探究百里香酚对山羊子宫内膜上皮细胞(goat endometrial epithelial cells,g EECs)的毒性作用,采用不同浓度的百里香酚作用于g EECs后,观测其对细胞生长曲线,细胞形态学变化,以及细胞膜结构完整性的影响。结果表明,百里香酚对EECs的毒性作用与其浓度呈剂量依赖效应(y=-0.008x+1.2202,R^2=0.9767),即随着药物浓度的增加细胞的增殖抑制率增大,药物对细胞的半数增殖抑制率(IC50)为100μg/m L。当50、100μg/m L的百里香酚作用于EECs时,g EECs的细胞核、细胞浆的形态均未产生明显变化,但当150μg/m L的百里香酚作用于g EECs时,细胞膜破裂,细胞死亡;而且药物浓度≥100μg/m L时细胞LDH的释放量显著高于正常细胞组,g EECs细胞膜的完整性受到破坏。百里香酚作用于山羊子宫内膜上皮细胞后呈现一定的毒性作用,且毒性作用和药物的浓度有关。百里香酚作用于g EECs的安全范围在0~100μg/m L,研究结果为百里香酚治疗奶牛等经济动物的炎症性疾病的临床用药浓度提供理论依据。In order to explore the cytotoxicity of thymol on goat endometrial epithelial cells （gEECs） , gEECs was treated by different concentrations of thymol. And the cell growth curve, the changes of cell morphology, the integrity of the cell membrane was detected. The results showed that thymol has a role in promoting proliferation of the gEECs. There was a dose- dependent effect between thymol toxicity to gEECs and concentrations （y=-0.008x＋ 1.2202, R^2= 0. 9767 ） , The inhibitory rate of cell proliferation increased with the increasing of the drug concentration; And 50% inhibitory rate of proliferation （IC50） of this drug on EECs is 100 μg/mL. When the 50 and 100 g/mL of thymol stimulated gEECs, the nuclear morphology and the cytoplasm of the gEECs cells didn＇t change significantly. But when 150 g/mL thymol treated gEECs, cell membrane rupture and cell would die ; When the concentration was more than 100 g/mL, the integrity of cell membrane can be damaged, promoting the release of LDH significantly compared with normal cells group. In summary, there was a certain toxicity of thymol on goat endometrial epithelial cells, and the toxicity related to concentration. The safe concentration of thymol is in the range of 0-100 g/mL. Which provided a theoretical basis not only for the clinical treatment on the inflammatory diseases of cows and other economic animal but also for the relevant experiments of thymol at the molecular level.

关 键 词：百里香酚 子宫内膜上皮细胞 细胞毒性 膜通透性 

分 类 号：S859.79[农业科学—临床兽医学]