一周岁内滩羊皮肤差异表达蛋白研究  

Study on Skin Differential Expression Proteins of Tan Sheep Within One Year Old

作　　者：杨佐青 陶金忠[1] 陈信[1] 马瑞华[1] 张鑫荣[2] 

机构地区：[1]宁夏大学农学院,银川750021 [2]宁夏盐池滩羊选育场,盐池751506

出　　处：《中国畜牧兽医》2017年第4期986-993,共8页China Animal Husbandry & Veterinary Medicine

基　　金：宁夏农业育种专项"滩羊品系选育"项目(2013NYYZ0402)

摘　　要：为研究一周岁内滩羊皮肤中的差异表达蛋白,本试验利用双向凝胶电泳(2-DE)技术分离了滩羊不同生长阶段皮肤蛋白,经考马斯亮蓝染色,用PDQuest 8.0软件检测并分析差异蛋白点,并经MALDITOF/TOF-MS/MS鉴定。对4个生长时期的蛋白图谱进行两两比对,共发现了19个差异蛋白点,质谱成功鉴定出15个蛋白点共13种蛋白,可能与滩羊皮肤生长调控有关的蛋白有14-3-3蛋白σ亚型(14-3-3protein sigma)、膜联蛋白A2(annexin A2)、角蛋白25(keratin 25)、微管蛋白α链(tubulin alpha chain)。对差异表达蛋白进行分析,发现这些差异表达蛋白可能与滩羊皮肤不同生长阶段的毛囊周期性变化有关。一周岁内滩羊皮肤差异蛋白的发现为滩羊皮肤的年变化规律研究奠定理论基础。In order to study different expressed proteins of one-year-old Tan sheep skin, two-dimensional gel eleetrophoresis （2-DE） was used to separate the Tan sheep skin proteins of different growth stages by Coomassie blue staining, with PDQuest 8.0 software to detect and analyze differences in protein spots, and were identified by MALDITOF/TOF-MS/MS. Protein maps of four growth stages were performed between pairwise, after testing found a total of 19 differentially expressed proteins, mass spectrometry successfully identified 15 protein spots and a total of 13 proteins, of which 14-3-3σ protein isoforms, annexin A2, keratin 25 and tubulin α chain might be related to growth regulation sheep skin. Those different expressed proteins might be associated with different stages of growth of Tan sheep skin follicles cyclical changes. The different pro- teins of one-year-old Tan sheep skin provided theoretical basis for annual variation of Tan sheep skin.

关 键 词：滩羊 皮肤 双向电泳 差异蛋白 

分 类 号：S826[农业科学—畜牧学]