藤茶提取物对小鼠生长性能、腹泻率及抗氧化性能的影响  

Effect of Ampelopsis grossedentata Extract on Growth Performance,Diarrhea Rate and Anti-oxidation Function in Mice

作　　者：陈丽[1] 孙云子[1] 

机构地区：[1]贵州师范大学生命科学学院,贵阳550025

出　　处：《中国畜牧兽医》2017年第4期1027-1031,共5页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省科技厅农业攻关计划项目(NZ[2013]3027);贵州省科学技术基金项目(J字LKS[2011]37);贵州师范大学资助博士科研项目"月桂醛单苷油酯对仔猪腹泻致病性大肠杆菌的抑杀作用"

摘　　要：试验旨在研究藤茶提取物对小鼠生长性能、腹泻率和抗氧化性能的影响。将90只小鼠随机分为空白组、对照组和试验Ⅰ、Ⅱ、Ⅲ组,空白组小鼠饲喂基础日粮,试验Ⅰ、Ⅱ、Ⅲ组分别在基础日粮中添加0.02、0.04、0.06mg/g体重的藤茶提取物,对照组在基础日粮中添加0.02mg/g体重的硫酸抗敌素。在试验第26天给小鼠灌服番泻叶,观察各日粮处理组小鼠腹泻情况。结果显示,各试验组和对照组小鼠试验末体重均高于空白组,且对照组显著高于空白组(P<0.05);添加藤茶提取物有提高小鼠生长性能和肝脏重的趋势,但与空白组相比差异不显著(P>0.05);试验Ⅱ、Ⅲ组和对照组小鼠腹泻率显著低于空白组和试验Ⅰ组(P<0.05);与空白组相比,对照组谷胱甘肽过氧化物酶(GSH-Px)活性显著提高(P<0.05),试验组也有提高趋势,但均未达显著水平(P>0.05);对照组的过氧化氢酶(CAT)活性显著高于试验Ⅱ组(P<0.05),各试验组之间超氧化物歧化酶(SOD)活性无显著差异(P>0.05)。以上结果表明,日粮中添加藤茶提取物能够能提高小鼠的生长性能及抗氧化性能,降低小鼠的腹泻率,可作为抗生素的替代品进一步研发。The effects of Ampelopsis grossedentata extract on growth performance, diarrhea rate and anti-oxidation function in mice were studied in this experiment. 90 mice were randomly divided into five groups： Blank group, experimental groups Ⅰ,Ⅱ,Ⅲ and control group. The mice in blank group were fed with basal diet, the experimental groups were added with 0.02, 0.04, 0.06 mg/（g·BW） of Ampelopsis grossedentata extract and the control group was added with colistin sulfate in basal diets, respectively. On the 26th day of the trial, the mice were administered folium senna and then record their diarrhea condition. The results showed that, the finial weights of mice in experimental and control groups were higher than that in blank group, and the difference between the blank and control groups were significant （P〈0.05）. Adding Ampelopsis grossedentata extract had a tendency to increase growth performance and liver weight, but the difference was not significant （P〉0.05）. The diarrhea rates in the control and experimental groups Ⅱ,Ⅲ were significantly lower than the blank and experimental group Ⅰ（P〈0.05）. Compared with the blank group, the activity of the serum glutathione peroxidase （GSH-Px） was significantly increased in control group （P〈0.05）, while the increasing trends were not significant in experimental groups （P〉0.05）. The activity of catalase （CAT） in control group was significantly higher than that in experimental group Ⅱ （P〈0.05）, and the serum superoxide dismutase （SOD） activities among the experimental groups were not significant （P〉0.05）. In conclusions, the growth performance and anti-oxidation function in mice could be increased, while the diarrhea rate could be decreased by adding the Ampelopsis grossedentata extract. It suggested that Ampelopsis grossedentata extract was expected to become a substitute for antibiotics in animal feeds.

关 键 词：藤茶提取物 小鼠 生长性能 抗氧化性能 

分 类 号：S816.7[农业科学—饲料科学]