四黄止痢颗粒中几种有效成分的薄层鉴别研究  

Study on the TLC Identification of Effective Components in the Sihuangzhili Granules

作　　者：贾纪萍[1] 熊玥 宋慧敏 李芳[1] 李正雄[1] 陈晓兰[1] 

机构地区：[1]江苏农牧科技职业学院,泰州225300 [2]江苏省兽药饲料质量检验所,南京210036

出　　处：《中国畜牧兽医》2017年第4期1233-1238,共6页China Animal Husbandry & Veterinary Medicine

基　　金：江苏农牧科技职业学院院级课题(NSFYB1404)

摘　　要：为建立和完善四黄止痢颗粒的质量标准,本试验采用薄层色谱法对四黄止痢颗粒中的黄连、黄柏、大黄、黄芩、板蓝根进行鉴别,对展开剂、展开温度、展开湿度、薄层板来源、薄层板批号、显色条件等因素进行考察。试验以环己烷-乙酸乙酯-异丙醇-甲醇-水-三乙胺(3∶3.5∶1∶1.5∶0.5∶1)为展开剂,置氨蒸气预饱和的展开缸内展开,置紫外光灯(365nm)下检视鉴别黄连、黄柏;以乙酸乙酯-丁酮-甲酸-水(5∶3∶1∶1)为展开剂展开,喷以2%三氯化铁乙醇溶液检测黄芩的存在;以正丁醇-冰醋酸-水(19∶5∶5)为展开剂展开,热风吹干后喷以茚三酮试液,在105℃加热检视鉴别板蓝根。试验结果表明,在考察条件下,目标物斑点清晰、无拖尾,阴性对照无干扰,方法专属性强,重现性好。In order to establish and perfect the quality standard of Sihuangzhili granules, this experiment was conducted to identify coptidis rhizome, cortex phellodendri, rhubarb, scutellaria baicalensis, and radix isatidis in Sihuangzhili granules. We used TLC identification method to investigate the factors such as the developing agent, the expansion temperature, the expansion of the humidity, the source of the thin layer, the batch number and the color developing conditions.In the experiment, the mixture of cyclohexane, ethyl acetate, isopropyl alcohol, methanol, water, and triethylamine （3∶3.5∶1∶1.5∶0.5∶1） as the developing agent, was put into the expansion cylinder with the pre-saturated ammonia vapor, and was used to identify coptidis rhizome and cortex phellodendri under the UV lamp（365 nm）;The mixture of ethyl acetate, butanone, formic acid and water （5∶3∶1∶1） as developing agent, sprayed with 2% ethanol solution of ferric chloride, was used to identify scutellaria baicalensis;At last, n-butanol, acetic acid, and water （19∶5∶5） as developing agent, dried and sprayed with indene ketone solution, heated at 105 ℃, was used to identify radix isatidis. The test results showed that under the test condition, the target spot was clear, with no tail, and the experiments were specifically suited in this test with strong specificity and good reproducibility.

关 键 词：四黄止痢颗粒 薄层色谱 鉴别 

分 类 号：R284.1[医药卫生—中药学]