生物材料在毛细管电泳分析中的应用  

Applications of bio-materials in capillary electrophoresis analysis

作　　者：李巧巧[1] 刘远平[2] 王凤琴[1] 夏之宁[1] 杨丰庆[1] LI Qiao-qiao LIU Yuan-ping WANG Feng-qin XIA Zhi-ning YANG Feng-qing(School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China Taiji Group Co., Ltd., Chongqing 401147, China)

机构地区：[1]重庆大学化学化工学院,重庆401331 [2]太极集团有限公司销售总公司,重庆401147

出　　处：《药物分析杂志》2017年第4期568-576,共9页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金项目(81202886;21275169)

摘　　要：毛细管电泳(CE)技术是研究生物分子之间或生物分子与其他小分子之间相互作用的主要方法之一,而基于生物大分子(多糖、核酸、蛋白质、酶)或生物大分子组装体(细胞膜、细菌、活细胞)与目标分子间的相互作用研究,已发展出潜在活性药物筛选、手性药物对映体拆分及物质检测(分子识别)等方面应用的CE方法。本文对不同种类生物材料包括多糖、核酸、蛋白质、酶、细胞膜、细菌和活细胞在CE中的应用进行综述,以期为生物材料在CE中进一步应用研究提供参考。Capillary electrophoresis （CE）is one of the primary methods for investigation of the interactions between bio-macromolecules or bio-macromolecules with other small molecules. Based on the interactions of bio-macromolecules （ such as polysaecharides, nucleic acids, proteins and enzymes ） and bio-macromolecule assemblies （ such as cell membrane, bacteria and living cells ） with the target molecules, several CE methods have been developed for bioactive compounds screening, chiral drugs enantiomer separation as well as molecular recognition detection. In this paper, the applications of diverse bio-materials in CE were reviewed, including polysaccharides, nucleic acids, proteins, enzymes, cell membrane, bacteria as well as living cells, to provide scientific basis for their further study.

关 键 词：生物材料 毛细管电泳(CE) 相互作用 活性成分筛选 对映体拆分 分子识别 

分 类 号：R917[医药卫生—药物分析学]