桑葚多糖T3-3分离及秀丽隐杆线虫抗衰老活性研究  

Optimization of the extracting parameters of mulberry polysaccharide and its anti-aging activity study

作　　者：张培丽[1] 陈雪香[1] 王群[1] 刘果[1] 张泳[1] 杨异[1] 曹庸[1] 

机构地区：[1]华南农业大学食品学院,广州510642

出　　处：《天津中医药大学学报》2017年第2期136-141,共6页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：广东高校国际科技合作创新平台项目(2013gjhz0003)

摘　　要：[目的]研究桑葚多糖T2提取工艺优化,并对其抗衰老活性进行分析。[方法]以秀丽隐杆线虫为模型,用寿命、移动力分析、吞咽频率、产卵量和肠脂褐质综合评价不同剂量范围的桑葚多糖(T3-3)抗衰老活性。通过正交和优化实验确定最优提取工艺条件为:提取温度100℃,提取时间2 h,提取次数3次,料液比1∶30,提取率最高达8.48%。[结果]抗衰老活性研究表明,150 mg/L的T3-3能增强秀丽隐杆线虫的抗衰老能力。[结论]该提取工艺1)延长了秀丽隐杆线虫的寿命,秀丽隐杆线虫平均寿命延长了10.38%(P<0.05),最大寿命延长了28.57%(P<0.05),50%存活率对应天数延长了0.91%。2)秀丽隐杆线虫吞咽频率增加,吞咽频率比对照组高18.78%。3)产卵量无统计学差异。4)减少秀丽隐杆线虫肠脂褐质的产生量,比对照组平均荧光强度低11.14%(P<0.05),实验末期,对照组的平均荧光强度增大了12.47%。[Objective] To study the optimization of the extracting parameters of mulberry polysaccharide and its anti-aging activity. [Methods] Usingel egans model, such as life, movement analysis, frequency of swallowing, spawning and intestinal brown quality comprehensive evaluation of different dose of T3-3 to study the anti-aging activity of itspurified components T3-3. [Results] The best extracted condition was： 100 ℃ of extraction temperate, 2 h of extraction time, 3 times, and 1∶30 of liquid ratio, the extracted rate would up to 8.48%. Anti-aging activity：T3-3 extend the average life span for 10.38%（P〈0.05）, the corresponded time of 50% survival rate extend 0.91%; the deglutition frequency is 18% higher than the control group; the egg laying amount have no significant difference; the intestinal lipofusin produced by nematode is reduced, 11.14%（P〈0.05） lower than the fluorescence intensity of control group.At the final stage of experiment, the average fluorescence intensity of control group increase 12.47%. [Conclusion]The results provide that the concentration of 150 mg/L, T3-3 has the best effect.

关 键 词：桑葚多糖提取工艺 抗衰老活性 研究分析 

分 类 号：R284[医药卫生—中药学]