苹果DREB转录因子家族全基因组鉴定与分析  

Genome-wide Identification and Analysis of the DREB Transcription Factor Gene Family in Apple

作　　者：沈兵琪 高晓宇[1,2] 王大玮[1,2] 周军[1,2] 邓浪[1,2] 周凡[1,2] 纪晴[1,2] 程慧[1,2] 

机构地区：[1]西南林业大学云南省高校林木遗传改良与繁育重点实验室,昆明650224 [2]西南林业大学西南山地森林保育与利用省部共建教育部重点实验室,昆明650224

出　　处：《西北植物学报》2017年第3期460-469,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家林业局948项目(2013-4-44);云南省林学一流学科建设经费;西南林业大学大学生创新基金(C16079)

摘　　要：DREB转录因子属于AP2/ERF转录因子家族,能够与DRE/CRT顺式作用元件特异性结合,调控与逆境应答基因的表达,因而在植物应对低温、干旱、高盐等逆境胁迫中发挥重要作用。该研究利用苹果全基因组数据,通过生物信息学手段鉴定苹果DREB转录因子家族成员,并分析DREB转录因子家族保守域特点与功能及表达情况。结果表明:从苹果全基因组中共鉴定出60个DREB转录因子家族成员,与拟南芥和水稻相比基本一致,通过引入拟南芥DREB基因进行系统发生分析,进一步可以将其细分为6个亚组;结构域和保守元件分析表明,DREB基因家族含有一个AP2保守结构域;染色体定位表明,苹果DREB基因分布于11条染色体上,部分基因存在串联复制现象;基因结构分析显示,该亚家族基因不含内含子。利用同源拟南芥RNA-Seq数据分析结果表明,DREB转录因子家族对低温、ABA调节等非生物胁迫具有调控作用,同时在DREB亚家族中每个亚组响应不同的非生物胁迫;通过分析DREB基因在不同组织中的表达情况,结果显示DREB基因在植物根部中的表达量最强,其次是叶。DREBtranscription factor belongs to the family of AP2/ERF transcription factors,which can specifically bind to DRE/CRT cis-acting elements,regulate the expression of stress response genes and play an important role in stress response to low temperature,drought and high salt stress.The members of DREBtranscription factor family were identified by bioinformatics analysis,and the conserved domain of DREBtranscription factor family was analyzed by using the whole genome data of apple,and the function and expression of DREBtranscription factor family were analyzed.The results showed that 60 members of DREBtranscription factor family were identified from the whole genome of apple,which was consistent with that of Arabidopsis and rice.The phylogenetic analysis of Arabidopsis DREB gene could be further subdivided into 6Subgroups;Domains and conserved elements analysis showed that the DREBgene family contained an AP2 conserved domain,chromosomal loci indicated that the DREBgene of apple was distributed on 11 chromosomes,and some genes had tandem duplication.Analysis of the gene structure showed that the subfamily gene did not contain introns.The analysis of RNA-Seq data of Arabidopsis thalianashowed that the DREBtranscription factor family regulates abiotic stress such as low temperature and ABA regulation,and each subgroup in the DREBsubfamily responds to different abiotic stresses.The expression of DREB gene in different tissues was analyzed.The results showed that the expression of DREBgene was the strongest in plant root,followed by leaf.

关 键 词：苹果 DREB 转录因子 生物信息学 同源表达 

分 类 号：Q786[生物学—分子生物学]