丹香冠心注射液HPLC指纹图谱及4种成分的含量测定  

HPLC Fingerprint of Danxiang Guanxin Injections and Determination of Four Components

作　　者：胡生俊 徐岳鑫 

机构地区：[1]宿迁市食品药品检验所,宿迁223800

出　　处：《药学与临床研究》2017年第2期99-101,共3页Pharmaceutical and Clinical Research

摘　　要：目的:建立丹香冠心注射液的HPLC指纹图谱,并测定其中4种有效成分的含量。方法:采用Agilent TC-C_(18)色谱柱;流动相为乙腈-0.05%三氟乙酸水溶液,梯度洗脱;检测波长288 nm;流速1.0 mL·min^(-1);柱温40℃。通过中药色谱指纹图谱相似度评价系统进行相似度评价。结果:标定了13个共有峰,10批样品的相似度均大于0.98;测定了10批样品中丹参素钠、原儿茶醛、迷迭香酸、丹酚酸B的含量,4种成分在标准曲线范围内线性关系良好(r>0.999),平均回收率均大于97%(n=6)。结论:该方法简便、快速、准确,可用于该品的质量控制。Objective： To establish an HPLC method for the fingerprint and determination of 4 active components in Danxiang Guanxin Injections. Methods： An Agilent TC-Ctg ccolumn was used, the mobile phase was acetonitrile-0.05% trifluoroacetic acid （ TFA） for gradient elution, the detection wavelength was 288 nm, the flow rate was 1 mL-min_1 and the column temperature was 40 %. The Similarity Evaluation System for Chromatographic Fingerprint of TCM was used to evaluate the similarity. Results： There were 13 common peaks; the similarity of 10 samples was over 0.98. The contents of danshensu -sodium, protocatechuic aldehyde, rosmarinic acid and salvianolic acid B were determined in the 10 samples. Within detection ranges of the 4 components, standard curves were linear （ r〉0.999）, average recovery rates were over 97% （ n=6）. Conclusion： The method is simple, rapid and accurate, which can be used for the quality control of Danxiang Guanxin Injections.

关 键 词：丹香冠心注射液 含量测定 指纹图谱 HPLC 

分 类 号：R927.2[医药卫生—药学]