基于脑缺血动物模型的针刺作用机制及选穴规律的挖掘研究  

Tentative exploration of acupoints＇ biology effects to the cerebral ischemia model

作　　者：黄满婷 雷蕾[1] 李海燕[1] Huang Manting Lei Lei Li Haiyan.(Institute of Information on Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China)

机构地区：[1]中国中医科学院中医药信息研究所,北京100700

出　　处：《国际中医中药杂志》2017年第4期333-338,共6页International Journal of Traditional Chinese Medicine

摘　　要：目的 运用数据挖掘方法 分析针刺实验文献中针刺对脑缺血动物模型的分子生物学作用机制的选穴规律.方法检索1978年1月-2015年12月中国期刊全文数据库（CNKI）、中国生物医学文献数据库（SinoMed）、万方科技数据库有关针刺动物实验的文献,筛选出与针刺对脑缺血动物模型基因蛋白表达影响相关的文献,人工提取文献中的穴位和基因蛋白指标,规范化处理后进行频次统计、关联规则及复杂网络计算分析并可视化展示.结果 获得符合研究范围的文献350篇;获得穴位79个,频次最高为百会;获得基因蛋白180个,频次最高为BCL2;关联规则计算得最常用组穴为足三里、曲池;复杂网络计算得与脑缺血损伤或康复机制关系较密切的基因/蛋白为热休克蛋白70、BCL2、CASP3、BCL2相关X蛋白.结论 针刺穴位可通过多条途径治疗缺血性脑血管疾病,穴位选择主要以督脉和阳明经的穴位为主.Objective The molecular biology mechanism of cerebral ischemia animal models be analyzed from acupuncture experimental literatures by using data mining methods as a tentative exploration. Methods Retrieve the literatures of acupuncture animal experiments during 1978-2015, and screen the literatures about acupuncture effect on cerebral ischemia animal model of gene protein expression. Acupoint and gene/protein targets were extracted and normalized, and then the frequency statistics, association rule, calculation of complex networks were analyzed and visually displayed.Results A total of 350 papers were included. Seventy-nine acupoints were summarized, and the most frequent acupoint wasbaihui; A total of 180 genes/proteins were found, and the most frequent genes/proteins was BCL2. The most common group acuppointszusanli and quchifor the association rule results. The results of complex networks analysis showed the HSP70, BCL2, CASP3, Bax were more closely associated with cerebral ischemia injury or rehabilitation mechanism. Conclusions The mechanism of acupuncture treatment for cerebral ischemia was that acupuncture adjusting ischemic brain damage and improve the nervous system disorders from many channels and multiple targets.

关 键 词：脑缺血 针刺穴位 基因表达 复杂网络 关联规则 

分 类 号：R245[医药卫生—针灸推拿学] R-332[医药卫生—中医临床基础]