HPLC法同时检测人血浆中奥卡西平及其代谢产物浓度  

Simultaneous determination of oxcarbazepine and its metabolite in human plasma by HPLC

作　　者：林奕沨[1] 黄成坷[1] 王哲[1] 朱光辉[1] LIN Yifeng HUANG Chengke WANG Zhe ZHU Guanghui(Department of Pharmacy, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, Chin)

机构地区：[1]温州医科大学附属第二医院药学部,温州325027

出　　处：《中国临床药学杂志》2017年第2期115-118,共4页Chinese Journal of Clinical Pharmacy

摘　　要：目的建立人血浆中奥卡西平(OXC)及其活性代谢产物10-羟基卡马西平(MHD)浓度的HPLC测定法。方法以卡马西平为内标,采用Agilent ZORBAX SB-C_(18)(100 mm×4.6 mm,5μm)色谱柱,流动相:水-乙腈(65∶35),流速1.0 m L·min^(-1),检测波长254 nm。结果 OXC与MHD血浆浓度在0.50~50.00 mg·L^(-1)内与峰面积之间线性关系良好(r分别为0.999 6和0.999 8);最低检测浓度0.50 mg·L^(-1),奥卡西平及其活性代谢产物相对回收率均为85%~115%,日内及日间RSD均<10%。结论本法简便、灵敏、准确,可用于奥卡西平及其活性代谢产物的临床血药浓度监测和药动学研究。AIM To develop an HPLC method for simultaneous determination of oxcarbazepine and its ac- tive metabolite 10-hydroxycarbazepine （MHD） in human plasma. METHODS Carbamazepine was chosen as the internal standard （IS）. The analytical column was packed with Agilent ZORBAX SB-CIs column （100 mm x4.6 mm, 5 μm）. The mobile phase consisted of acetonitrile and water （65： 35）. The flow rate was 1.0 mL · min-1 The UV detection wavelength was 254 nm. RESULTS The excellent liner relationship of oxcarbazepine and MHD were both range from 0.5 to 50 mg·L-1 （ r = 0. 999 6, r = 0. 999 8）. The limit determination was 0.5 mg·L-1. The relative recovery of oxcarbazepine and its active metabolite were both range from 85% to 115%. RSDs of intra-day and inter-day precision were both 〈 10%. CONCLUSION The method is simple, sensitive and accu- rate and could be used for therapeutic drug monitoring and pharmacokinetic study.

关 键 词：高效液相色谱法 奥卡西平 活性代谢产物 血药浓度 

分 类 号：R969[医药卫生—药理学]