罗哌卡因惊厥对大鼠海马细胞凋亡及学习记忆能力的影响  

Effects of ropivacaine-induced convulsion on cell apoptosis of hippocampus and the ability of learning and memory in adult

作　　者：欧益金[1] 徐世元[2] 张庆国[2] 

机构地区：[1]广州医科大学附属脑科医院、广州市惠爱医院麻醉科,510370 [2]南方医科大学珠江医院麻醉科,广州510282

出　　处：《国际麻醉学与复苏杂志》2017年第4期333-337,共5页International Journal of Anesthesiology and Resuscitation

摘　　要：目的研究罗哌卡因惊厥EDso致单次惊厥对大鼠海马细胞凋亡及学习记忆能力的影响。方法110只SD大鼠按随机数字表法分为生理盐水组（Ns组，35只）和罗哌卡因组（R组，75只）。R组大鼠腹腔注射罗哌卡因惊厥ED。致单次惊厥。分别在R组惊厥后2h、6h、24h、3d、7d的5个时点，NS组和R组各取5只大鼠海马组织冠状切片行Bax、Bcl-2蛋白免疫组化检测，显微镜下观察单位面积内Bax和Bcl-2阳性表达细胞的形态和数目。在R组惊厥后8、39d两个时间点，Ns组和R组大鼠各进行1次Morris水迷宫实验（定位航行实验和空间搜索实验）。结果成年大鼠腹腔注射罗哌卡因惊厥ED。致单次惊厥后，惊厥潜伏期为（9．3±3．7）S，惊厥持续时间为（7．2±4．6）s。与NS组比较，R组Bax蛋白表达在惊厥后2h时即升高，24h时达峰后回落，7d时仍高于NS组（P〈0．05）；Bcl-2蛋白表达在惊厥后2h时反应滞后（P〈0．05），6h时升高（P〈0．05），24h时达峰后回落（尸≮0．05），7d时恢复正常（P〉O．05）。惊厥近期R组逃避潜伏期和空间搜索成绩明显差于NS组（P〈O．05），惊厥远期R组逃避潜伏期和空间搜索成绩与NS组比较差异无统计学意义（P〉0．05）。结论腹腔注射罗哌卡因惊厥ED50致单次惊厥可引起大鼠海马组织细胞凋亡增加、惊厥近期学习记忆能力下降，但对惊厥远期学习记忆能力影响不大。Objective To investigate the influence of ropivacaine-induced convulsion on cell apoptosis of hippocampus and the ability of learning and memory in adult rats. Methods One hundred and ten SD adult rats were randomly divided into two groups： group NS （n=35） and group R （n=75）. Group R were i.p injected of convulsion EDs0 of ropivacaine and rats undergoing convulsion were selected for experiment. Group NS were i.p. injected of NS. At 2 h, 6 h, 24 h, 3 d, 7 d after convulsion, 5 rats in each group were chosen, then their hippocampus tissues were embedded with paraffin and siced up for immunohistochemistry experiments. The positive expressions of Bax and Bcl-2 in hippocampus were calculated with microscope. Ten rats in each group were chosen and the effects of ropivacaine-induced convulsion on spatial memory and learning ability were observed in 8 day and 45 day after convulsion. Results Convulsion latency and persistence time of group R were（9.3±3.7） s and （7.2±4.6） s. The expression of Bax of group R increased at 2 h after convulsion, reached the top at 24 h and then declined, which was still higher than group NS in 7 d. The expression of Bcl-2 of group R increased at 6 h （P〈0.05） after convulsion, reached the top at 24 h （P〈0.05） and then down to normal in 7 d （P〈0.05）. Group R had less scores than group NS in the results of EL and CT （P〈0.05） in 8 d after convulsion, while there was no statistical significance in 45 d （P〉0.05）. Conclusions Convulsion induced by i.p. convulsion EDs0 of ropivacaine increased ceils apoptosis of hippocampus and reduced the ability of learning and memory in earlier period after convulsion, but it had little impact in longer period after convulsion.

关 键 词：罗哌卡因 惊厥 凋亡 学习 记忆 大鼠 

分 类 号：R614[医药卫生—麻醉学]