贵州野生白刺花种质资源AFLP遗传多样性研究  

Genetic Diversity of Wild Sophora davidii Germplasm in Guizhou Province Based on AFLP Markers

作　　者：龙忠富[1,2] 侯扶江[1] 张建波[3] LONG Zhong-fu HOU Fu-jiang ZHANG Jian-bo(College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020,China Guizhou Pratacultural Institute, Guiyang 550006, China Sichuan Academy of Grassland Science, Chengdu 611700, China)

机构地区：[1]兰州大学草地农业科技学院,甘肃兰州550006 [2]贵州省草业研究所,贵州贵阳550006 [3]四川省草原科学研究院,四川成都611700

出　　处：《中国草地学报》2017年第2期105-110,共6页Chinese Journal of Grassland

基　　金：国家牧草产业技术体系(CARS-35-37);贵州省科技厅资助项目"优质饲用灌木晴隆白刺花高效利用关键技术研究与示范"(黔科合NY字[2012]3040号)

摘　　要：利用AFLP标记研究了11份贵州野生白刺花的遗传变异情况,结果表明:(1)8对AFLP引物共扩增出719条条带,平均扩增89.9条条带,平均多态信息含量为0.46,多态性条带共613条,平均多态性条带76.6条,多态性条带占85.4%,表明各材料间出现了较大的遗传变异。(2)供试材料样本间平均相似系数为0.701,白刺花的遗传相似性系数较小,说明贵州区域的野生材料出现了较大的遗传变异。(3)聚类分析发现所有供试材料可以分为4大类,聚类结果混乱,主成分分析发现材料间分布分散,缺乏规律性。(4)遗传距离与地理距离的Mantel检验发现白刺花的遗传变异与其地理距离相关性差。Eleven accessions of wild Sophora davidii Germplasms in Guizhou Province were studied with AFLP markers. The results showed that： 1） Eleven pairs AFLP markers generated 719 bands and mean bands were 89.9, and the number of polymorphic bands were 613 and mean polymorphic bands were 76.6, 85.4% were polymorphic bands. There was rich genetic diversity among the tested materials of Sophora davidii. 2） The mean similarity coefficient was 0. 701, it means that there was great genetic variation in wild Sophoradavidii Germplasm. 3） All tested materials were clustered into four groups by UPGMA den- drogram, the result of cluster analysis was irregular, 4）The genetic variation of Sophora davidii was less associated with geographic distance using Mantel test.

关 键 词：白刺花 AFLP标记 聚类分析 主成分分析 

分 类 号：S541[农业科学—作物学]