牛煅烧骨/Ⅰ型胶原复合骨修复材料制备及评价  

Preparation and Evaluation of True Bone Ceramic/Type Ⅰ Collagen Composite Bone Repair Materials

作　　者：雷雄心 李赛娜 康跻耀[3] 彭公泽 张贵锋[3] 

机构地区：[1]北京中医药大学中药学院,北京100010 [2]山东农业大学食品学院,山东泰安271018 [3]中国科学院过程工程研究所,北京100190 [4]南方医科大学珠江医院,广东广州510280

出　　处：《过程工程学报》2017年第2期389-394,共6页The Chinese Journal of Process Engineering

基　　金：国家高技术研究发展计划(863)基金资助项目(编号:2014AA022109);广东省自然科学基金资助项目(编号:2014A030312013)

摘　　要：将牛煅烧骨(TBC)和Ⅰ型胶原(CoL)按不同质量比混合,经戊二醛交联、冷冻干燥等处理后制得复合支架,研究了不同材料比例及胶原交联对材料性能的影响,分析了支架材料的理化性能,通过CCK-8法评价了细胞在支架上的增殖情况.结果表明,所制TBC/CoL支架具有多孔结构,平均孔隙率为93.5%,杨氏模量为4.36±0.21 MPa;复合支架中TBC在CoL上发生了化学吸附,CoL结构保持完整;MC3T3-E1细胞在支架上培养24 h在支架表面生长良好,增殖情况与对照组无明显差别(P>0.05).The true bone ceramic （TBC） and type I collagen （COL） were mixed with different mass ratios and collagen crosslinked by glutaraldehyde. The heterogeneous mixture was freeze-dried and the dehydrated state was obtained as the scaffold. The effects of material and cross-linking of collagen on the scaffold properties were investigated. The physical and chemical properties of the scaffolds were analyzed. The proliferation of the cells on the scaffold was evaluated by the CCK-8 method. The results showed that the prepared TBC/CoL scaffolds had a porous structure with an average porosity of 93.5% and a Young＇s modulus of 4.36~0.21 MPa. TBC in the composite scaffolds were chemically adsorbed on the CoL matrix and the CoL structure in the matrix remained intact. MC3T3-E1 cells were cultured on the scaffold, after 24 h of culture, the cells grew well. The proliferation of cells on the scaffold was not significantly different from the control group （P〉0.05）.

关 键 词：牛煅烧骨 Ⅰ型胶原 骨组织工程 支架 

分 类 号：R318.08[医药卫生—生物医学工程]