新加良附颗粒对人胃癌细胞SGC-7901移植瘤裸鼠血清IL-23、IL-21水平的影响  

Effects of Modified Liang-Fu Granule on Level of IL-21 and IL-23 in Serum of SGC-7901 Tumor-bearing Nude Mice

作　　者：吴晓勇[1] 陈信义[2] 宋延平[3] 赵林涛[3] 田杰[4] 周义浪[4] 王云龙[1] 陈广雷[1] 侯丽[2] 

机构地区：[1]贵阳中医学院第一附属医院,贵州贵阳550001 [2]北京中医药大学东直门医院,北京100700 [3]陕西省中医药研究院,陕西西安710003 [4]贵阳中医学院第二附属医院,贵州贵阳550003

出　　处：《亚太传统医药》2017年第8期13-15,共3页Asia-Pacific Traditional Medicine

基　　金：贵州省科学技术厅项目(黔科合J字[2011]2296号);贵州省科学技术厅;贵阳中医学院科学技术联合基金(黔科合中药字[2010]LKZ7034);2010年贵阳中医学院博士启动基金;北京中医药大学科学科研基地建设项目北京市重点实验室(中医内科学)(2011-JDJS-02)

摘　　要：目的:观察新加良附颗粒对人胃癌细胞SGC-7901移植瘤裸鼠血清IL-23、IL-21水平的影响,初步探讨其可能的作用机制。方法:60只裸鼠造模成功后随机分为空白组、5-Fu组、新加良附中剂量+5-FU组(联合给药组)及新加良附颗粒高、中、低剂量组。新加良附颗粒高、中、低剂量组给药剂量分别为10g·d^(-1)·kg^(-1)、5g·d^(-1)·kg^(-1)、2.5g·d^(-1)·kg^(-1);5-FU组给药剂量为17mg·d^(-1)·kg^(-1);空白组给予等体积生理盐水,联合给药组剂量为新加良附颗粒中剂量(5g·d^(-1)·kg^(-1))联合应用5-FU(17mg·d^(-1)·kg^(-1))。连续给药10天,进行眼球取血并处死小鼠,采用ELISA法检测血清IL-23、IL-21的含量。结果:联合给药组、5-FU组、新加良附高、中、低剂量组裸鼠血清中IL-23、IL-21含量较空白对照组均明显降低(P<0.01);与新加良附大剂量组、5-FU组比较,联合给药组血清IL-23含量差异无统计学意义(P>0.05),与新加良附大剂量组、5-FU组比较,联合给药组血清IL-21含量差异具有统计学意义(P<0.05)。结论:新加良附颗粒联合5-FU具有抑制人胃癌细胞SGC-7901移植瘤裸鼠血清中TH17细胞相关细胞因子IL-21、IL-23的表达,其可能通过调节TH17细胞相关细胞因子的表达影响胃癌的发生与发展。Objective：To observe the effects of modified liang-fu granules （mLFG）on level of IL-23 and IL-21 in serum of SGC-7901 tumor bearing nude mice. Discussion the possible mechanism of mLFG on gastric cancer. Methods ： Establish the tumor-bearing nudemice models with human gastric cancer cell line SGC-7901, which were divided randomLy into six groups：blank group, 5-FU group, combination group and mLFG in high, medium,low dose groups. The mice were treated for 10 days and then detecting the level of IL- 23 and IL-21 in serum of SGC-7901 tumor bearing nude mice with the method of ELISA. Results：The levels of IL-23 and IL-21 in 5-FU group,combination group, mLFG in high, medium, low dose groups were significantly lower than those in blank group （P〈 0.01）. The levels of serum IL-23 in the combination group were compared with that of mLFG in high dose group and the 5-FU group,the difference was not significant（P〉0.05）. The levels of serum IL-21 in the combination group were compared with that of mLFG in high dose group and the 5-FU group,the difference was significant（P〈0.05）. Conclusion：The mLFG combined with 5- FU maybe inhibit the expression of IL-23 and IL-21 in the serum of human gastric cancer cell SGC-？901 xenograft tumor bearing nude mice,which may affect the development of gastric cancer by regulating the expression of TH17 cell related cytokines.

关 键 词：人胃癌细胞SGC-7901 新加良附颗粒 IL-23 IL-21 

分 类 号：R285.5[医药卫生—中药学]