阿霉素增强CIK细胞抗多发性骨髓瘤细胞的机制研究  

Mechanism of adriamycin enhanced CIK cells cytotoxicity against multiple myeloma cells

作　　者：姚阳[1] 周民[2] 卢绪章[3] 姜玉[3] 岑岭[3] 张修文[3] 杨建和[3] 

机构地区：[1]泰州职业技术学院,江苏泰州225300 [2]常州市第三人民医院,江苏常州213000 [3]南京医科大学附属常州第二人民医院,江苏常州213000

出　　处：《东南大学学报（医学版）》2017年第1期20-23,共4页Journal of Southeast University(Medical Science Edition)

基　　金：2014年泰州市科技支撑社会发展计划(指导性)项目(201433);常州市卫生局青年科技项目(QN201203);常州市卫生局重大项目(ZD201311)

摘　　要：目的:探讨阿霉素增强细胞因子诱导的杀伤性(CIK)细胞抗多发性骨髓瘤细胞的机制。方法:用阿霉素处理骨髓瘤细胞株U266以及患者骨髓瘤细胞,流式细胞仪(FCM)检测细胞表面NKG2D配体(MICA/B和ULBPs)表达的变化及CIK细胞对U266细胞杀伤作用的变化。结果:阿霉素处理后U266细胞表面MICA/B表达明显升高(P=0.002),而ULBPs的表达无明显变化。阿霉素亦能够诱导患者骨髓瘤细胞表面MICA/B的表达增加。CIK细胞对阿霉素处理过的U266细胞杀伤作用明显增加(P=0.01),这种杀伤作用可以被抗NKG2D抗体部分抑制(P=0.03)。结论:化疗药物诱导骨髓瘤细胞表面MICA/B表达的增加,从而增强CIK细胞对骨髓瘤细胞的杀伤作用。Objective： To explore the mechanism of adriamycin enhanced CIK cells cytotoxicity against multiple myeloma cells. Methods： Multiple myeloma cell line U266 and the cells from patients with multiple myeloma were treated with adriamycin,the expression of NKG2D ligands （MICA/B and ULBPs） were analyzed by flow cytometry （FCM）. The cytotoxicity of CIK cells against U266 cell was detected by flow cytometry. Results： The expression of MICA/B on multiple myeloma cell line U266 was up- expression of ULBPs had no changed. The expression regulated after treated with adriamycin （ P = 0. 002）, however of MICA/B on primary plasma cells was also up-regulated af- ter treated with adrimycin. The cytotoxicity of CIK cell against U266 was significantly increased by treated with adri- amycin （ P = 0.01 ）, and the enhancing cytotoxicity was partly blocked by anti- NKG2D Abs （ P = 0.03 ）. Conclu- sion ：The cytotoxicity of CIK cells against multiple myeloma an enhance by treated with adriamycin through Up-reg- ulating of MICA/B.

关 键 词：NKG2D配体 细胞因子诱导的杀伤性细胞 骨髓瘤细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]