长江中下游麦区小麦品种籽粒硬度及puroindoline基因等位变异的分子检测  

Allelic Variation of puroindoline Alleles in Bread Wheats from Lower-Middle Reaches of the Yangtze River

作　　者：王化敦[1] 高春蕾[2] 张鹏[1] 张瑜[1] 张平平[1] 马鸿翔[1] WANG Huadun GAO Chunlei ZHANG Peng ZHANG Yu ZHANG Pingping MA Hongxiang(Jiangsu Academy of Agricultural Sceinces/Jiangsu Provincial Key Laboratory for Agrobiology, Nanjing,Jiangsu 210014, China College of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China)

机构地区：[1]江苏省农业科学院/江苏省农业生物学重点实验室,江苏南京210014 [2]南京农业大学农学院,江苏南京210095

出　　处：《麦类作物学报》2017年第4期438-444,共7页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(31501819);国家现代农业产业技术体系项目(CARS-3);江苏省自然科学基金项目(BK20161375)

摘　　要：为了明确长江中下游麦区小麦籽粒硬度及puroindoline基因型的分布,以该麦区105份小麦育成品种为材料,利用单籽粒硬度仪(SKCS)测定其籽粒硬度,利用分子标记检测和基因序列分析鉴定puroindoline基因的等位变异。结果表明,在长江中下游麦区历年育成的小麦品种中软质麦比例较高,占52.4%,硬质麦和混合麦分别占38.1%和9.5%;硬质麦和混合麦中存在Pinb-D1b、Pina-D1b和Pinb-D1p三种变异类型,突变频率分别为29.5%、10.5%和3.8%。To elucidate the grain hardness index and the distribution of hardness-related gene in the lower-middle reaches of the Yangtze River wheat region, 105 wheat varieties widely collected from this region were used in this study. Grain hardness was examined by a Single Kernel Characterization System （SKCS）. Molecular detection and sequencing analysis were used to identify puroindoline al- leles. The results showed that soft wheat accounts for 52.4%, while hard and mixed wheat account for 38. 1% and 9. 5%, respectively. Three mutant puroindoline alleles of Pinb-Dlb, Pina-Dlb and Pinb-Dlp were detected in hard and mixed wheat. Of them, the frequency of Pinb-Dlb was the high- est, with the percentage of 29. 5%, followed by Pina-Dlb and Pinb-Dlp, with the percentage of 10.5% and 3.8% , respectively.

关 键 词：普通小麦 籽粒硬度 PUROINDOLINE基因 

分 类 号：S512.1[农业科学—作物学] S331