葛根素纳米晶的制备工艺及其表征  

Characteristics and Process Technology of Peurarin Nanocrystals

作　　者：杜俊锋[1] 涂亮星[1] 胡凯莉[1] 奉建芳[1,2] 

机构地区：[1]上海中医药大学,上海201203 [2]桂林医学院,广西桂林451004

出　　处：《湖南中医药大学学报》2017年第4期369-372,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家"973"计划项目(2009CB930300);上海市优秀技术带头人计划项目(13XD1425300);上海市科委纳米专项项目(11nm0506700)

摘　　要：目的研究葛根素纳米晶的制剂处方、制备工艺及其对粒径的影响,并对葛根素纳米晶进行表征。方法采用高压均质法制备葛根素纳米晶,以粒径为指标,考察稳定剂、药辅比、投料量、制备温度等参数对纳米晶制备的影响。在此基础上,考察干燥工艺对葛根素纳米晶固化的影响。采用扫描电镜(SEM)及透射电镜(TEM)进行葛根素及纳米结晶形态学的观察。结果采用聚维酮K30作为稳定剂、药辅比为1∶2、制备温度43℃以上时,可制得粒径为20~50 nm的葛根素纳米晶。投药量对纳米晶粒径没有显著性影响,投药量为10%(w/v)时,采用700 bar 5次的高压均质条件即可制得所需的纳米晶。冷冻干燥可以用于纳米晶的干燥。将葛根素制成纳米结晶之后,其外观由不规则状态变为圆整的球形。结论所确定的葛根素纳米晶制剂处方与制备工艺条件可行,可有效地制备100 nm以下的葛根素纳米晶。Objective In this study, the production, process technology and effects of particle size of puer-arin nanocrystals were investigated, and the puerarin nanocrystals were characterized. Methods High pressure homogenization （HPH） was used for formula and production studies. Particle size as an indicator, we studied the effects of stabilizers, drug/stabilizer ration, drug input and production temperature on the production of peurarin nanocrystals, and then drying method were introduced to study the solidification of nanocrystals. We introduced scanning electron microscopy （SEM） and transmission electron microscopy （TEM） for morphologic observation for crystalline study. Results In the production study, puer-arin nanocrystal with diameter of 20~50 nm could be produced after using PVP K30 as stabilizer, with drug/stabilizer ratio 1：2, temperature above 43℃.The amounts of puerarin input has no significant influence on nanocrystals production. In poilet scaled experiments, nanocrystals with puerarin input of 10% （w/v） were produced at 700 bar for 5 cycles, and the nanocrystals pre-pared were solidified by freezing drying and spray drying. We found that after transferred puerarin into nanocrystals, the irregu-lar shape for puerarin was changed to spherical shape for nanocrystals. Conclusion The formula and production processes esta-bilished were reliable and repeatability, the puerarin nanocrystals with diameter below 100 nm could be prepared effectively.

关 键 词：葛根素 纳米晶 制备工艺 粒径 表征 

分 类 号：R283[医药卫生—中药学]