不同规格全血制备富血小板血浆的最佳离心条件研究  

The Optimal Centrifugation for Preparation of Platelet-rich Plasma from Different Volumes of Whole Blood

作　　者：王淑君[1] 赵广超[1] 栾建凤[1] 朱培元[1] 

机构地区：[1]江苏省南京军区南京总医院输血科,210002

出　　处：《临床输血与检验》2017年第2期119-122,共4页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：全军重大课题(No.ANJ13J001);南京军区医院科研基金(No.2015049)资助

摘　　要：目的研究不同规格全血分离制备富血小板血浆(PRP)的最佳离心条件。方法共采集54例健康献血者的新鲜全血,根据离心次数和离心条件分为两次离心组(A、B、C三种分离方法)和三次离心组(D、E、F三种分离方法)共6组,每组含200、300和400 ml全血各6袋。检测每组制备PRP的血小板计数和红细胞混入量,计算血小板回收率并进行比较。结果两次离心法所制备PRP的血小板回收率最高,但红细胞混入量较多,其中200 ml全血采用方法A最佳(即初次850g离心8 min,第2次4 650 g离心5 min),300 ml全血为方法B(初次1 000 g离心6 min,第2次4 650 g离心5 min),400 ml全血为方法C(初次1 220 g离心5 min,第2次4 650 g离心6 min)。三次离心法与二次离心法相比,红细胞混入量明显减少(P<0.05),但血小板回收率有所降低。结论不同规格全血制备PRP应采用不同的离心条件,两次离心法适用于制备新鲜PRP或血小板胶,而三次离心法更适合冰冻或冻干PRP或血小板胶。Objective To explore the optimal centrifugation conditions for preparation of platelet-rich plasma （PRP） from different volume of whole blood. Methods Fifty-four bags of freshly collected whole blood from volunteer donors were divided into 6 groups according to centrifugal steps and centrifugal conditions, including 3 two-step （ separation method A , B , and C） and three-step centrifugation groups （ separation method D, E, and F） , with 3 bags of 200 ml, 300 ml, and 400 ml whole blood for each group. The platelet count （PLT） and red blood cell count （RBC） of PRP in each group were determined, then PLT recovery was calculated and compared. Results Compared to three-step centrifugation groups, the PRP prepared in two-step centrifugation group had higher PLT recovery and RBC count. The highest PLT recovery in PRP from 200 ml whole blood was prepared by method A （e.g, 850 × g for 8 min, then 4 650 X g for 6min） , 300 ml whole blood by method B （ e.g, 1000 × g for 6 min, then 4650 × g for 6 min） , and 400ml whole blood by method C （ e.g, 1220× g for 5 min, then 4650× g for 6 min） . The RBC count of PRP in each three-step centrifugation group was decreased significantly （P 〈0.05） . Compared with two-step centrifugation group, the PLT recovery in three-step centrifugation group was slightly decreased. Conclusion Different centrifugation conditions should be used for preparation of PRP from different volumes of whole blood . The two-step centrifugation method is suitable for preparation of fresh PG or platelet gel, while the three-step centrifugation method is appropriate for frozen and lyophilized PRP or platelet gel.

关 键 词：富血小板血浆 无偿献血者 制备 

分 类 号：R331.143[医药卫生—人体生理学]