液相色谱法测定工作场所空气中对苯二胺  

Determination of p-Phenylene diamine in the air of workplace by high-performance liquid chromatography

作　　者：刘保峰[1] 张健[1] 张明[1] 刘静[1] 张万超[1] 张宇帅[1] 赵淑岚[1] 曾强[1] 

机构地区：[1]天津市疾病预防控制中心职业病预防控制所,天津300011

出　　处：《公共卫生与预防医学》2017年第2期22-24,共3页Journal of Public Health and Preventive Medicine

基　　金：国家卫生和计划生育委员会职业卫生标准项目(20140704)

摘　　要：目的建立测定工作场所空气中对苯二胺(PPD)的高效液相色谱法。方法工作场所空气中PPD用装有5.0m L甲醇吸收液的冲击式采样管,直接进样,经Eclipse XDB-C18液相色谱柱分离,紫外检测器检测,以保留时间定性,峰高或峰面积定量。结果 PPD质量浓度在0.50~5.00mg/L浓度范围内具有良好的线性关系,相关系数为0.999 03;按照采集空气样品45L计算,最低检出浓度为0.004mg/m^3;批内和批间精密度为0.66%~2.14%和1.01%~4.17%;平均采样效率为99.4%;样品在4℃条件下至少可存放3d。结论该方法灵敏度高,精密度好,操作简单,能有效避免PPD易于氧化问题,适用于工作场所空气中PPD的测定。Objective To establish the detection method for p-Phenylene diamine（PPD） in the air of workplace by highperformance liquid chromatography.Methods The air sample of PPD was quantitatively detected by the method of external standard after a series of steps collection with shocking absorber tube containing 5.0mL methyl alcohol solution followed by direct sampling separated with a Eclipse XDB-C18 column and tested with a ultraviolet detector.Results The PPD standard curve showed a good linear relationship under the mass concentration of 0.5-5.0mg/L（correlation coefficient=0.999 03）.The air samples were calculated according to 45.0L the minimum average desorption efficiency was 0.004mg/m^3;the within-run precision of the average desorption efficiency was 0.66%-2.14% and the between-run precisions was 1.01%-4.17%;the average sampling efficiency was 99.4%;The samples could be stably kept at 4℃ for 4d at least.Conclusion This method could be used for detection PPD in the air with high sensitivity precision and simple operation.

关 键 词：色谱法 液相 空气 工作场所 对苯二胺 

分 类 号：R134.4[医药卫生—劳动卫生]