化瘀解毒中药对梗阻性肾病大鼠肾脏类肝素酶表达的调控作用  

Effect of Stasis-resolving and Toxin-removing Chinese Medicine on Heparanases Expression in Obstructive Nephropathy Rats

作　　者：马国平[1] 贾彦存 郝娟[3] 王佳磊[4] 杨帆[3] 张宇[3] 梁文杰[3] 

机构地区：[1]河北医科大学第一医院,石家庄050030 [2]河北省安平县医院 [3]河北中医学院河北省中西医结合肝肾病证重点实验室 [4]河北医科大学,石家庄050017

出　　处：《河北中医药学报》2017年第2期5-7,共3页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省科技计划项目:No.152777208

摘　　要：目的:观察化瘀解毒中药对梗阻性肾病大鼠肾脏类肝素酶(Hpa)表达的影响,以探索其拮抗炎症损伤的机制。方法:采用单侧输尿管结扎手术复制梗阻性肾病模型;采用real-time PCR技术检测肾组织Hpa mRNA的表达;采用SABC法检测肾组织Hpa蛋白的表达。结果:与假手术组比较,模型组大鼠肾组织Hpa mRNA及蛋白表达上调(P<0.01);与模型组比较,中药组肾组织Hpa表达下降(P<0.01)。结论:化瘀解毒中药可下调梗阻性肾病大鼠肾脏Hpa表达从而减轻炎症损伤。Objective： to observe the effect of stasis-resolving and toxin-removing Chinese medicine on heparanases （ Hpa） expres-sion in obstructive nephropathy rats so to explore its mechanism of anti-inflammatory injury. Methods ： Take unilateral ureteral obstruc-tion to duplicate obstructive nephropathy models; take real-time technique to detect Hpa mRNA expression; take SABC to detect Hpa protein expression. Results： compared to sham operation group, Hpa mRNA expression of model group increased （ P 〈0. 01） ; com-pared to model group, Hpa expression of medicine group decreased （ P 〈 0. 01 ） . Conclusion： Stasis-resolving and toxin-removing Chinese medicine can decrease the Hpa expression in obstructive nephropathy rats and so as to lighten the inflammatory injury.

关 键 词：化瘀解毒中药 类肝素酶 梗阻性肾病 炎症损伤 

分 类 号：R285.5[医药卫生—中药学]