天祝白牦牛MSTN基因编码区克隆及生物信息学分析  

Cloning and bioinformatics analysis of MSTN gene of Tianzhu white yak

作　　者：张丽[1] 刘丽霞[1] 李强子[1] 陈红[1] 

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030

出　　处：《浙江农业学报》2017年第4期618-624,共7页Acta Agriculturae Zhejiangensis

基　　金：西北民族大学引进人才科研项目(xbmuyjrc201316);西北民族大学生命科学与工程学院众创空间扶持项目;西北民族大学中央高校基本科研业务费专项资金项目(31920140077)

摘　　要：为探讨天祝白牦牛MSTN基因的分子结构特征,通过PCR扩增和测序技术以及生物信息学分析工具对天祝白牦牛MSTN基因进行克隆、测序及相关生物信息学分析。结果表明,天祝白牦牛MSTN基因编码区序列全长1 128 bp,编码375个氨基酸残基;天祝白牦牛MSTN基因编码蛋白分子量42.55 ku,理论等电点为6.14;生物信息学预测发现,天祝白牦牛MSTN蛋白是不稳定的亲水蛋白,mRNA二级结构的最小自由能为-1 135.16 k J·mol^(-1),蛋白质二、三级结构均是以无规卷曲和延伸链为主的混合型蛋白。天祝白牦牛MSTN基因的成功克隆为进一步研究牦牛MSTN的遗传特性和生理机制奠定了基础。In order to elucidate the structure of M577V gene, the complete MSTN gene sequence of Tianzhu white yak was amplified and analyzed through PCR, cloning, sequencing and the bioinformatics tools. The results showed that the sequence of MSTN gene was 1 128 bp in length and encoded 375 amino acids with a molecular weight of 42. 55 ku and pi of 6. 14. Bioinformatics prediction found that MSTN protein of Tianzhu white yak is unstable hydrophilic protein and mRNA secondary structure of minimum free energy is - 1 135. 16 kJ＊mol 1. Protein secondary and terti-ary structure were based on extended strand and random coil-based mixed-type protein. The results laid a foundation for further studying on the genetic characteristics and physiological mechanisms of MSTN.

关 键 词：天祝白牦牛 MSTN基因 克隆 生物信息学 

分 类 号：S823.85[农业科学—畜牧学]