24个中外猪种(群)的AFLP多态性及其群体遗传关系  

Studies of Population Genetic Relationships Among 24 Chinese and Exotic Pig Breeds Using AFLP Analysis

作　　者：任军[1] 黄路生[1] 艾华水[1] Gary Evens 高军[1] 陈克飞[1] 丁能水[1] 邓素华[1] 

机构地区：[1]江西农业大学江西省动物生物技术重点实验室,南昌330045 [2]DepartmentofPathology,UniversityofCambridge

出　　处：《Acta Genetica Sinica》2002年第9期774-781,T002,共9页

基　　金：国家自然科学基金项目 (批准号 3 9860 0 63 )资助~~

摘　　要：利用 12对AFLP引物组合检测了 19个中国地方猪种 (群 )、1个培育猪种和 4个欧美引进猪种混合基因组DNA的遗传变异 ,根据AFLP分析结果计算了 2 4个猪种 (群 )间的遗传相似系数 ,据此构建了UPGMA聚类关系图。结果表明 ,AFLP标记有着很高的多态检测效率 (Ai) ,平均每个引物组合检测到 17.3个多态标记 ,非常适合于猪种(群 )遗传多样性分析和品种鉴定 ;中国地方猪种与欧美引进猪种间的遗传分化十分明显 ,两类猪群间的亲缘关系较远 ;南昌白猪与大白猪、铅山黑猪与玉山黑猪有着极近的亲缘关系 ,分别与其育成历史、地理分布和RAPD分析结果相一致。另外还对部分猪种 (群 )的聚类分析结果与其形态学。A total of 12 AFLP primer combinations were used to detect genetic variation of pooled DNA in a sample of 19 Chinese native pig breeds, 1 cultivated pig breed and 4 European and American pig breeds. The genetic similarity coefficient of 24 pig beeds was calculated from AFLP data, UPGMA cluster analysis was also performed. The 12 primer combinations generated more than 1000 bands, of which 208 bands were polymorphic, 17.3 polymorphic markers were detected by one primer combination on the average. Thirteen putative breed specific bands were produced in the pooled DNA of 8 pig breeds. The cultivated pig breed and 4 exotic pig breeds were clustered into one group, while 19 Chinese native pig breeds were gathered into the other group in the UPGMA tree. The result indicated that AFLP analysis had high assay efficiency index (Ai) and provided a valuable tool for assaying genetic diversity and breed characterization in pigs. Chinese native pig breeds and exotic pig breeds show remarkable genetic differentiation, which had farther genetic relationships. Nanchang White pig and Large White pig, Yushan Black pig and Yanshan Black pig hadintimate genetic relationships with each other respectively, which were consistent with its breeding history, geographical distribution and RAPD analysis results. In addition, the reasons for cluster results of some pig breeds from AFLP data were not consistent with morphology, geographical distribution and existing classification were discussed.

关 键 词：中国地方品种 国外引进品种 猪种 AFLP多态性 群体遗传关系 

分 类 号：S828.2[农业科学—畜牧学]