转化生长因子对巨噬细胞抗新生隐球菌功能的影响  

Effects of transforming growth factor on the function of macrophage against Cryptococcus neoformans

作　　者：李颖芳[1] 桑军军 李娟[1] 姜伟伟[1] 廖万清[1] 潘炜华[1] 

机构地区：[1]上海长征医院皮肤病与真菌病研究所全军真菌病重点实验室第二军医大学附属长征医院皮肤科,上海200003

出　　处：《中国真菌学杂志》2017年第2期65-68,共4页Chinese Journal of Mycology

基　　金：973项目(2013CB531606);国家自然基金(31270180)

摘　　要：目的研究转化生长因子对巨噬细胞吞噬和杀伤新生隐球菌(Cryptococcus neoformans)功能的影响。方法采用TGF-β干预体外活化巨噬细胞吞噬和杀伤实验,分别观察巨噬细胞吞噬率和巨噬细胞内隐球菌生长情况,采用Griess regent试剂盒检测巨噬细胞NO(Nitric oxide)生成量,并比较TGF-β干预下巨噬细胞NO生成量变化。结果 TGF-β干预后巨噬细胞吞噬率下降了43.9%,差异有统计学意义(P<0.05)。巨噬细胞在转化生长因子干预下NO生成量增加,胞内隐球菌负荷降低,差异有统计学意义(P<0.05)。结论体外细胞实验中,在TGF-β干预下活化巨噬细胞的趋化作用和吞噬能力受抑制,但巨噬细胞合成NO量增加,杀伤隐球菌能力增强。Objective To investigate the phagocytosis and killing ability of macrophage under TGF-βintervention.Methods Using TGF-βintervene phagocytosis and killing assay of activated macrophages in vitro.Phagocytic index of macrophage and the intracellular growth of Cryptococcus neoformans was observed respectively.Nitric oxide（NO）production was detected using the Griess regent kit,and then comparing the changes of NO production in macrophages under the TGF-βintervention.Results The phagocytic index with the treatment of TGF-βdecreased by 43.9%,significantly lower than control group（P〈0.05）.While the production of NO increased and the intracellular fungal burden significantly declined under the intervention of TGF-β（P〈0.05）.Conclusion Chemotaxis and phagocytosis of activated macrophage were inhibited under the intervention of TGF-βin vitro,but the NO production increased and killing ability enhanced.

关 键 词：新生隐球菌 转化生长因子 巨噬细胞 

分 类 号：R379.5[医药卫生—病原生物学]