水稻线粒体DNA中与雄性不育有关特异片段的克隆及序列分析  

Cloning and Sequencing of Fragments Associated with Cytoplasm Male Sterility of Rice

作　　者：杨剑波[1] 汪秀峰[1] 赵成松[1] 向太和[1] 李莉[1] 

机构地区：[1]安徽省农业科学院农业部水稻遗传育种重点开放实验室,合肥230031

出　　处：《Acta Genetica Sinica》2002年第9期808-813,共6页

摘　　要：该项研究利用RAPD技术 ,对野败型、矮败型和BT型细胞质雄性不育系、保持系 ,基因组DNA进行PCR扩增 ,分离到 6条不育系特有的扩增片段 ,并对野败型、矮败型不育系共有的片段进行了Southern分析、DNA序列分析和SCAR验证 ,该片段全长 1879bp ,包含 6个开放阅读框架和 8对重复序列。BLAST分析表明 ,该片段部分区域与Elytrigiaelongata、小麦线粒体tRNA Asp基因上游一段序列同源。Mitochorndrial DNA (mtDNA),nuclear DNA (nDNA), and genomic DNA (gDNA) were individually extracted from Zhenshan 97A, Xieqingzao A and A 23, which were cytoplasmic male sterile (wild abortive, dwarf abortive and BT), and from Zhenshan 97B, Xieqingzao B and BT, which were maintainers lines. Each of them was analyzed with 500 random primers by RAPD. Six fragments specific to male sterile lines were amplified. A fragment, PWH 17, associated with wild and dwarf abortive type cytoplasmic male sterility was analyzed by Southern blotting and sequencing, and also tested by SCAR. It was 1879bp in length, which contained 6 open reading frames and 8 repeated sequences. BLAST search revealed that partial sequence in PWH 17 showed high similarity to the upstream sequence of tRNA Asp gene in Elytrigia elongate (GenBank accession number U14335) and wheat (X13243). The similarity ratios were 97% and 100%. It was inferred that the possible position of PWH 17 in rice (wild abortive) could be located between tRNA Asp and Cox Ⅱ, which will be discussed further.

关 键 词：水稻 线粒体 DNA 雄性不育 特异片段 克隆 序列分析 SCAR RAPD 

分 类 号：S511.02[农业科学—作物学]