溶脲脲原体的尿素酶基因变异及其在生物分型中的意义  

Studies on the variations among urease genes of Ureaplasma urealyticum and its role in the biovar diversity

作　　者：彭清忠[1] 郭兆彪[2] 朱厚础[1] 杨瑞馥[2] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《微生物学免疫学进展》2002年第3期1-5,共5页Progress In Microbiology and Immunology

摘　　要：应用PCR技术成功地从溶脲脲原体 14个血清型中获得尿素酶基因片段。经分析发现各序列间高度保守 ,但也存在一定程度的变异。据此绘制的系统进化树可将 14个血清型分为两大群 ,与现在两个生物型划分的唯一区别是将属于生物 1型的血清型 3归于生物 2型。在 14个型的序列中 。The fragments of urease genes from different serotypes of Ureaplasma urealyticum were obtained by PCR and were analysed by software clustalw and treeview after being sequenced,which revealed that there were variations in urease genes of different serotypes and reflected the phylogenetic relationship of two biovars.Based on the diversity of urease genes,serotypes 1,6,14 of the parvo biovar could be distinguished from T 960 biovar,but serotype 3 was more similar to the T 960 biovar than to serotpyes 1,6,14.These mismatched nucleotide acids among 14 amplicons might be signatures bases of different serotypes,and be of interest for identification at the serotype level.

关 键 词：生物群 血清型 生物分型 溶脲脲原体 尿素酶基因变异 

分 类 号：R375.2[医药卫生—病原生物学]