Genotypic,size and morphological diversity of virioplankton in a deep oligomesotrophic freshwater lake(Lac Pavin,France)  

作　　者：Jonathan Colombet Agnès Robin Télesphore Sime-Ngando 

机构地区：[1]Laboratory Microorganisms:Oenome and Environment,Clermont University Blaise Pascal,UMR CNRS 6023,24 avenue des Landais,BP 80026,F-63171 Aubiere,France [2]CIRAD,UMR Eco&Sols,2 place Viala,34060 Montpellier Cedex 1,France

出　　处：《Journal of Environmental Sciences》2017年第3期48-59,共12页环境科学学报（英文版）

基　　金：supported by a PhD Fellowship from the Grand Duchédu Luxembourg(BFR04/047,Ministry of Culture,High School,and Research);supported by the French National Program ACI/FNS“ECCO”(VIRULAC research grant awarded to TSN,coordinator);the French ANR Program“Biodiversité”(AQUAPHAGE research grant to TSN,PI)

摘　　要：We examined changes in morphological and genomic diversities of viruses by means of transmission electronic microscopy and pulsed field gel electrophoresis （PFGE） over a nine-month period （April-December 2005） at four different depths in the oligomesotrophic Lac Pavin. We found that the majority of viruses in this lake belonged to the family of Siphouiridae or were untailed, with capsid sizes ranging from 30 to 60 nm, and exhibited genome sizes ranging from 15 to 45 kb. On average, 12 different genotypes dominated each of the PFGE fingerprints. The highest genomic viral richness was recorded in summer （mean = 14 bands per PFGE fingerprint） and in the epilimnion （mean = 13 bands per PFGE fingerprint）. Among the physico-chemical and biological variables considered, the availability of the hosts appeared to be the main factor regulating the variations in the viral diversity.We examined changes in morphological and genomic diversities of viruses by means of transmission electronic microscopy and pulsed field gel electrophoresis （PFGE） over a nine-month period （April-December 2005） at four different depths in the oligomesotrophic Lac Pavin. We found that the majority of viruses in this lake belonged to the family of Siphouiridae or were untailed, with capsid sizes ranging from 30 to 60 nm, and exhibited genome sizes ranging from 15 to 45 kb. On average, 12 different genotypes dominated each of the PFGE fingerprints. The highest genomic viral richness was recorded in summer （mean = 14 bands per PFGE fingerprint） and in the epilimnion （mean = 13 bands per PFGE fingerprint）. Among the physico-chemical and biological variables considered, the availability of the hosts appeared to be the main factor regulating the variations in the viral diversity.

关 键 词：VirusLac PavinDynamicMorphological diversity Genomic diversity 

分 类 号：Q939.4[生物学—微生物学]