An optimized two-step derivatization method for analyzing diethylene glycol ozonation products using gas chromatography and mass spectrometry  

作　　者：Ran Yu Lei Duan Jingkun Jiang Jiming Hao 

机构地区：[1]State Key Joint Laboratory of Environment Simulation and Pollution Control,School of Environment,Tsinghua University,Beijing 100084,China [2]State Environmental Protection Key Laboratory of Sources and Control of Air Pollution Complex,Beijing 100084,China

出　　处：《Journal of Environmental Sciences》2017年第3期313-321,共9页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(nos.21422703 and 41227805);the National Key Basic Research and Development Program of China(no.2013CB228505)

摘　　要：The ozonation of hydroxyl compounds （e.g., sugars and alcohols） gives a broad range of products such as alcohols, aldehydes, ketones, and carboxylic adds. This study developed and optimized a two-step derivatization procedure for analyzing polar products of aldehydes and carboxylic acids from the ozonation of diethylene glycol （DEG） in a non-aqueous environment using gas chromatography-mass spectrometry. Experiments based on Central Composite Design with response surface methodology were carried out to evaluate the effects of derivatization variables and their interactions on the analysis. The most desirable derivati- zation conditions were reported, i.e., oximation was performed at room temperature overnight with the o-（2,3,4,5,6-pentafluorobenzyl） hydroxyl amine to analyte molar ratio of 6, silylation reaction temperature of 70℃, reaction duration of 70 min, and N,O-bis（trimethylsilyl）- trifluoroacetamide volume of 12.5 μL. The applicability of this optimized procedure was verified by analyzing DEG ozonation products in an ultrafine condensation particle counter simulation system.The ozonation of hydroxyl compounds （e.g., sugars and alcohols） gives a broad range of products such as alcohols, aldehydes, ketones, and carboxylic adds. This study developed and optimized a two-step derivatization procedure for analyzing polar products of aldehydes and carboxylic acids from the ozonation of diethylene glycol （DEG） in a non-aqueous environment using gas chromatography-mass spectrometry. Experiments based on Central Composite Design with response surface methodology were carried out to evaluate the effects of derivatization variables and their interactions on the analysis. The most desirable derivati- zation conditions were reported, i.e., oximation was performed at room temperature overnight with the o-（2,3,4,5,6-pentafluorobenzyl） hydroxyl amine to analyte molar ratio of 6, silylation reaction temperature of 70℃, reaction duration of 70 min, and N,O-bis（trimethylsilyl）- trifluoroacetamide volume of 12.5 μL. The applicability of this optimized procedure was verified by analyzing DEG ozonation products in an ultrafine condensation particle counter simulation system.

关 键 词：Gas chromatography-massspectrometryResponse surface methodologyCentral Composite DesignDiethylene glycol ozonation 

分 类 号：X831[环境科学与工程—环境工程] X513