血小板聚集率与富血小板血浆血小板浓度关系的研究  

Research on Relation of Platelet Aggregation Rate with Platelet Concentration in Platelet-Rich Plasma'

作　　者：韩松梅[1] 廖文丽[2] 于帅 

机构地区：[1]中国人民解放军总医院海南分院内分泌科,海南三亚572013 [2]海南医学院热带医学与检验医学院,海南海口571199 [3]解放总医院海南分院检验中心,海南三亚572013

出　　处：《中国实验血液学杂志》2017年第2期582-586,共5页Journal of Experimental Hematology

基　　金：解放军总医院科技创新苗圃基金(16KMM43)

摘　　要：目的:探讨富血小板血浆(PRP)中血小板浓度对血小板聚集率的影响,初步确定诱聚剂二磷酸腺苷(ADP:5.0和15μmol/L)和花生四烯酸(AA:500.0μg/ml)对血小板浓度的允许范围。方法:选取健康查体人群枸橼酸钠抗凝外周血标本72例,每12例标本混合为1组,分6组。将各组标本混合后,分别制备不同水平的血小板浓度,利用海伦娜血小板聚集分析仪分别检测AA和ADP诱导下的6组不同血小板浓度水平的血小板聚集率。结果:当诱聚剂浓度升高,不同浓度的血小板聚集率会随之增高。随着血小板浓度升高,AA和ADP(15μmol/L)诱导的血小板聚集率均呈现先快速上升而后平缓的趋势,其中当Plt浓度低于200×10~9/L时,AA诱导的血小板聚集率随着血小板浓度的降低出现陡降,而ADP诱导的血小板聚集率随着血小板浓度的升高稍平缓些。当Plt>200×10~9/L,AA 500μg/ml和ADP 15μmol/L诱导时,不同浓度Plt的血小板聚集率变化平缓、波动较少,较稳定。结论:进行血小板聚集率检测时,AA和ADP诱聚剂对应的适宜Plt浓度应大于200×10~9/L。Objective： To explore the effect of platelet concentration in platelet-rich plasma （PRP）＇ on platelet aggregation rate, to determine the allowed range of inducer adenosine diphosphate （ ADP： 5.0 μmol/L, ADP： 1.5μmol/L） and arachidonic acid （AA： 500.0 μg/ml） for platelet concentration in platelet aggregation rate. Methods： The venous blood samples of 72 healthy persons with citrate antieoagulation were selected and divided into groups, every 12 cases were mixed for each group. After the samples were mixed, different levels of platelet concentrations were prepared, and platelet aggregation rate of 6 groups with different platelet concentrations by ADP and AA inducer were detected by Helena platelet aggregation analyzer respectively. Results ： When the concentration of the inducer increased, the platelet aggregation rate of different platelet concentrations increased. With the platelet concentration increased, AA and ADP （ 15μmol/L） induced platelet aggregation rate showed a rapid increase at first and then showed slowing, when the Plt concentration is less than 200× 10^9/L, AA induced platelet aggregation rate decreased with the steep drop, and ADP induced platelet aggregation rate increased slightly slowly along with platelet concentration increasing. When platelet count lower than 200 × 10^9/L and AA 500 μg/ml and ADP 15 μmol/L were used for induction, the changes of platelet aggregation rate of different concentrations of platelet was slow, less volatile and more stable. Conclusion： When the platelet aggregation rate was detected, the appropriate platelet concentration of AA and ADP inducing agents should be greater than 200 × 10^9/L.

关 键 词：血小板聚集率 富含血小板血浆 血小板浓度 二磷酸腺苷 花生四烯酸 

分 类 号：R331.143[医药卫生—人体生理学]