High Five昆虫细胞系的致瘤性研究  

Tumorigenicity of High Five insect cell line

作　　者：汪伟[1] 周俊明[1] 王小敏[1] 何孔旺[1] 温立斌[1] 倪艳秀[1] 

机构地区：[1]江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室国家兽用生物制品工程技术研究中心,南京210014

出　　处：《中国实验动物学报》2017年第2期186-189,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家公益性行业(农业)科研专项(201303046-10-3);江苏省自主创新基金[CX(14)2045;ZX(15)1003)]

摘　　要：目的检测High Five昆虫细胞系对BALB/c裸鼠致瘤性情况,以确保其作为疫苗生产细胞株的安全性。方法将裸鼠随机分为5组,分别为High Five基础细胞库细胞试验组、High Five最高限制代次细胞试验组、HEp-2细胞阳性对照组、CEF细胞阴性对照组和不作任何处理的空白对照组,用细胞悬液背部皮下接种裸鼠。3周和12周后对裸鼠解剖及病理组织学检查,其是否有肿瘤形成。结果阳性对照HEp-2细胞组裸鼠接种后3周注射部位形成结节,病理组织学检查为鳞状细胞癌;阴性对照CEF细胞组和High Five细胞组均无结节形成,接种后12周经解剖用病理组织学检查,均无肿瘤形成。结论基础代次和最高限制代次High Five细胞均不具有致瘤性,可安全地应用于疫苗生产。Objective To observe the tumorigenicity of High Five insect cell line in Balb/c nude mice, and make sure the safety of the cells when used in vaccine production. Methods Balb/c nude mice were randomly divided into 5 groups ： the basic cell bank of High Five group, the highest limited passages of High Five group, HEp-2 cell group as positive control, CEF cell group as negative control, and blank control. Except of the blank control, cell suspension was injected sub- cutaneously into the nude mice in the different groups, respectively. At 3 and 12 weeks after injection, anatomical observation and histopathologic examination were performed to detect the tumor formation. Results At 3 and 12 weeks after injection, the tumorigenicity study results showed that no tumor developed at the transplantation site in the blank control group, negative group, and High Five groups. Histopathological examinations also showed no abnormality in these groups. Otherwise, squa- mous cell carcinoma was developed in the positive group at 3 weeks after injection. Conclusions High Five cells of basic cell bank and highest limited passages are not tumorigenic, and can be used in vaccine production safely.

关 键 词：致瘤性 裸鼠 HIGH Five细胞 

分 类 号：Q95-33[生物学—动物学]