8年生转基因库安托杨外源基因转移及对土壤微生物数量影响的检测  

Exogenous Gene Transformation of 8-Year-Old Multi-gene Transgenic Populus × euramericana‘Guariento'and Its Influence on Soil Microbial Quantity

作　　者：朱文旭[1] 丁昌俊[1] 张伟溪[1] 张冰玉[1] 黄秦军[1] 褚延广[1] 苏晓华[1] 

机构地区：[1]林木遗传育种国家重点实验室,国家林业局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学研究》2017年第2期349-353,共5页Forest Research

基　　金：国家“863”国家高技术研究发展计划项目(2013AA102703);林业公益性行业科研专项经费重大项目(201004004)

摘　　要：[目的]评价转基因库安托杨可能引起的生态风险。[方法]利用PCR扩增研究外源基因水平转移情况,利用稀释平板法研究土壤中细菌、真菌和放线菌数量。[结果]电泳结果显示:林下杂草混合样品和土壤微生物DNA样品中均未出现目的基因片段。转基因株系和非转基因株系的非根际土壤中可培养的细菌、真菌和放线菌数量在杨树的不同生长期出现一定的变化,但是这种变化没有明显的规律。[结论]8年生转基因库安托杨未出现外源基因水平转移,也未对土壤微生物的数量产生显著影响。To evaluate the possibility of the ecological risk caused by multi-gene transgenic Populus×euramericana ‘Guariento’.[Method]The target genes were amplified by PCR, and the amounts of three species of rhizosphere soil microbe, including bacteria, actinomycetes and fungi, were counted.[Result]No target PCR product was observed in all samples analyzed. The amounts of bacteria, fungi and actinomycetes in non-rhizosphere soil between transgenic poplar and non-transgenic poplar did have some changes in different growing period, but no obvious regularity was found in these changes themselves.[Conclusion]The result suggested that no horizontal transfer of transgene occurred, and the gene transformation did not significantly affect the soil microbial quantity.

关 键 词：转多基因杨树 基因水平转移 土壤微生物 生态安全性 

分 类 号：S718.46[农业科学—林学]