2种甘蔗病害胁迫果蔗NBS类抗病基因同源序列的表达分析  

Expression analysis of NBS resistance gene analogs from chewing cane under two sugarcane diseases stress

作　　者：付瑜华[1] 贺尔奇 雷石富[1] 李向勇[1] 张正学[1] 卢加举[1] 

机构地区：[1]贵州省亚热带作物研究所,贵州兴义562400

出　　处：《广东农业科学》2017年第2期118-123,共6页Guangdong Agricultural Sciences

基　　金：贵州省科技厅联合基金(黔科合J字LKN[2013]03号);贵州省亚热带作物科技创新人才基地建设经费(黔人领发[2016]22号)

摘　　要：在眼斑病和轮斑病病原菌侵染下,分析果蔗NBS类抗病基因同源序列在侵染初期的表达情况,为后续克隆关键抗病基因、研究抗病机理提供理论依据。已克隆的7条果蔗RGAs可视为来源不同的3个基因片段。将2种病原菌分别针刺接种到感病基因型黔糖3号和抗病基因型黔糖5号叶片后,3条果蔗RGAs在2种病害侵染初期均受到不同程度的上调,RGA12478对两种病害的响应最为迅速,RGA5的表达量最高,尤其在轮斑病菌的胁迫下,RGA5在胁迫后12 h的表达量大约分别是同时间点RGA12478和RGA36表达量的16倍和6倍。因此,RGA5可认为是果蔗在抵御轮斑病和眼斑病侵染初期发挥主要作用的抗病基因片段,后续应结合RACE技术获得基因全长,作为关键抗病候选基因研究其功能和在外源信号因子作用下的表达情况,全面了解该抗病基因的抗病机理。Expression analysis of NBS resistance gene anologs （ RGA ） from chewing cane under infection of Drechslera sacchari （ Butler ） Subram. and Jain and Leptosphaeria sacchari Breda de Haan, respectively, were performed in order to provide theoretical basis for cloning disease resistance genes and studying disease resistance mechanism, seven cloned chewing cane RGAs were considered as 3 gene fragments. The 2 pathogenic bacteria were inoculated into leaves of the susceptible genotype QT 3 and the disease resistant genotype QT 5 using needle puncturing method respectively, all 3 chewing cane RGAs were up-regulated in the initial infection. The response of RGA12478 to the two diseases was the most rapid while the expression of RGA5 was the highest. Especially under the infection of L. sacchari, the expression of RGA12478 at 12h was about 16 and 6 times of RGA5 and RGA36 expression at the same time point respectively. Therefore, RGA5 was considered to be a key disease resistance gene fragment from chewing cane playing a major role in resisting the infection of D. sacchari and L. sacchari in the initial infection stage respectively. To study the overall disease resistance mechanism of RGA5, follow-up research should includ cloning the full-length gene using RACE technology, analysis of gene fimction and expression level under exogenous signal factor treatments.

关 键 词：果蔗 眼斑病 轮斑病 抗病基因同源序列 表达分析 

分 类 号：S432.23[农业科学—植物病理学]