变换波长法测定复方樟脑搽剂中樟脑和苯酚的含量  

Determination of camphor and phenol in compound camphor liniment by wavelength conversion HPLC

作　　者：盖静[1] 盖丽[2] 张烨[1] 

机构地区：[1]兰州军区联勤部药品仪器检验所,甘肃兰州730050 [2]成都军区机关医院,四川成都610011

出　　处：《中兽医医药杂志》2017年第2期42-44,共3页Journal of Traditional Chinese Veterinary Medicine

摘　　要：目的:建立变换波长的方法测定复方樟脑搽剂中樟脑和苯酚的含量。方法:采用高效液相色谱法测定复方樟脑搽剂中樟脑和苯酚的含量,色谱柱Shim-pack VP-ODS(150 mm×4.6 mm,5μm),以甲醇-水(65∶35)为流动相,流速:1.0 mL/min,柱温:25℃,进样量:20μL,检测波长范围:0~5 min为270 nm,5~10 min为288 nm。结果:樟脑和苯酚的线性范围分别为0.051 40~1.028 00 mg/mL(r=0.999 9)与0.052 32~1.046 00 mg/mL(r=0.999 9),平均回收率分别为97.82%(RSD=0.9%)和98.09%(RSD=1.9%)。结论:本法操作简便、结果准确、重现性好,可用于复方樟脑搽剂的质量控制。Objective： A wavelength conversion HPLC method for the determination of camphor and phenol in compoundcamphor liniment was established. Methods： HPLC method was used for the determination Of camphor and phenol in compoundcamphor liniment with Shim-pack VP-ODS column and methanol-water （65：35） as mobile phase. The flow rate was 1.0 mL·min-land column temperature was 25℃, sample size： 20 IxL. The detection wavelength was set at 270 nm （0-5 min） and 288 nm （5-10min）. Results： The linear range were 0.051 40-1.028 00 mg·mL-1 （r=0.999 9） and 0.052 32-1.046 00 mg/mL （r=-0.999 9）, forcamphor and phenol respectively. The corresponding average recovery rate was 97.82% （RSD=1.5%） and 98.09% （RSD=I.8%）.Conclusion： The method is simple, accurate, and with good reproducibility, which can be used as quality control for compoundcamphor liniment.

关 键 词：高效液相色谱法 复方樟脑搽剂 樟脑 苯酚 含量测定 

分 类 号：S853.7[农业科学—临床兽医学]