急性一氧化碳中毒大鼠肝脏微循环灌注变化及高压氧暴露的影响  

Changes in hepatic microcirculation perfusion after acute carbon monoxide poisoning and effects of hyperbaric oxygen in rats

作　　者：王丛[1] 高春锦[1] 梁芳[1] 李茁[1] 杨晶[1] 马丽娜[2] 

机构地区：[1]首都医科大学附属北京朝阳医院高压氧科,北京100020 [2]暨南大学附属郑州医院高压氧科

出　　处：《中华航海医学与高气压医学杂志》2017年第1期28-31,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的 应用激光多普勒血流成像仪观察急性一氧化碳中毒（acute carbon monoxide poisoning, ACOP）大鼠肝微循环灌注值（perfusion unit, PU）水平，以及高压氧（hyperbaric oxygen, HBO）暴露对肝微循环的影响。方法 将90只Wistar大鼠用抽签法随机分成9组：正常对照组、一氧化碳染毒第1、3、7、12天组（CO1组、CO3组、CO7组、CO12组）、染毒＋高压氧治疗第1、3、7、12天组（HBO1组、HBO3组、HBO7组、HBO12组），应用激光多普勒血流成像仪检测大鼠肝脏及肠道黏膜微循环血流灌注值。结果 在正常对照组、CO1组与HBO1组中，肝微循环灌注值比肠微循环灌注值显著下降，差异有统计学意义（P〈0.05），但二者变化趋势一致；与CO1组相比HBO1组、HBO3组、HBO7组、HBO12组肝微循环灌注值显著提高（P〈0.05），但与CO12相比HBO12组肝微循环差异无统计学意义（P〉0.05）。结论 应用激光多普勒血流成像仪检测能够客观反映肝微循环灌注变化，ACOP可导致肝微循环灌注减少，HBO治疗能够改善肝微循环灌注障碍。Objective To observe changes in hepatic microcirculation perfusion after acute carbon monoxide poisoning and effects of hyperbaric oxygen （HBO） on hepatic microcirculation by using laser Doppler blood perfusion imager.Methods Ninety Wistar rats were randomly divided into 9 groups： the control group, the CO exposure group which was subdivided into the CO exposure day 1, 3, 7 and 12 groups （or the CO day 1 group, the CO day 3 group, the CO day 7 group and the CO day 12 group）, the CO exposure ＋ HBO group which was subdivided into CO exposure ＋ HBO therapy day 1, 3, 7 and 12 groups （or the HBO day 1 group, HBO day 3 group, HBO day 7 group and HBO day 12 group）. Levels of hepatic and intestinal mucosa microcirculation perfusion were detected by using laser blood perfusion imager.Results In the control group, the CO day 1 group and the HBO day 1 group, the level of hepatic microcirculation perfusion was significantly lower than that of intestinal mucosa microcirculation perfusion. Statistical significance could be seen when comparisons were made between them（P〈0.05）, however, changes of the two were identical. The level of hepatic microcirculation perfusion in the CO day 1 group was obviously increased, as compared with those of the HBO day 1, 3, 7 and 12 groups（P〈0.05）. However, there was no statistical significance when comparisons were made between the CO day 12 group and the HBO day 12 group（P〉0.05）.Conclusions Laser Doppler blood perfusion imager could detect changes in the level of hepatic microcirculation perfusion. Acute carbon monoxide poisoning could obviously decrease the level of hepatic microcirculation perfusion, but HBO treatment could help to improve hepatic microcirculation disturbance.

关 键 词：急性一氧化碳中毒 高压氧 肝脏 微循环 激光多普勒血流成像仪 

分 类 号：R595.1[医药卫生—内科学]