高效液相色谱-氢化物发生-原子荧光联用技术测定饮用水中四种形态砷  

Determination of four arsenic species in drinking water by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry

作　　者：宋冠仪 梅勇[1] 商律 姬晓宇 刘佩佩[1] 宋世震[1] 

机构地区：[1]武汉科技大学公共卫生学院职业危害识别与控制湖北省重点实验室,湖北武汉430065

出　　处：《环境与职业医学》2017年第4期350-353,共4页Journal of Environmental and Occupational Medicine

摘　　要：[目的]建立高效液相色谱-氢化物发生-原子荧光(HPLC-HG-AFS)联用技术测定生活饮用水中四种形态砷的方法。[方法]通过高效液相色谱实现水中形态砷的分离,原子荧光进行定性及定量。对硼氢化钾质量分数、盐酸体积分数、氢氧化钠质量分数、缓冲液质量浓度和溶液p H值等实验条件进行优化,再运用到实际样本检测中。[结果]三价砷[As(Ш)]、一甲基砷酸(MMA)、二甲基砷酸(DMA)和五价砷[As(V)]的检出限分别为0.36、0.69、0.39和2.34μg/L,四种形态砷的标准曲线相关系数均大于0.999,平均加标回收率为95.7%~107.9%,相对标准偏差为0.23%~9.31%。[结论]采用HPLC-HG-AFS技术测定饮用水中不同形态砷,检出限低,精密度好,结果准确可靠。[ Objective ] To establish a method for determination of four arsenic species in drinking water by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry （HPLC-HG-AFS）. [ Methods ] Arsenic speciation in water was separated by high performance liquid chromatography, and qualitatively and quantitatively determined by atomic fluorescence spectrometry. Experimental conditions were optimized including potassium borohydride mass fraction, hydrochloric acid volume fraction, sodium hydroxide mass fraction, buffer concentration, and solution pH. Then the method was applied to actual water samples. [ Results ] The detection hmits of arsenic （Ⅲ）, monomethylarsenic acid （MMA）, dimethylarsenic acid （DMA）, and arsenic （V） were 0.36, 0.69, 0.39, and 2.34 μg/L, respectively. All correlation Coefficients were greater than 0.999. The standard recovery rates were 95.7%-107.9%. The relative standard deviations were 0.23%-9.31%. [ Conclusion ] HPLC-HG-AFS is featured by low detection limit and high precision with accurate and reliable testing resuhs for determination of arsenic speciation in drinking water.

关 键 词：高效液相色谱 原子荧光 砷 形态 饮用水 

分 类 号：R115[医药卫生—公共卫生与预防医学]