外源硅对PEG胁迫下小麦幼苗生长及抗氧化酶活性的影响  

Effects of exogenous silicon on plant growth and activity of anti-oxidative enzymes in wheat seedlings under drought stress

作　　者：郑世英[1] 郑建峰[2] 徐建[3] 郑芳[1] 李士平[1] 张乃芹[1] 于凌春[1] 耿建芬[1] 

机构地区：[1]德州学院生态与园林建筑学院,山东德州253023 [2]德州学院经济与管理学院,山东德州253023 [3]德州学院物理与电子学院,山东德州253023

出　　处：《干旱地区农业研究》2017年第2期74-78,共5页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金(31271667)

摘　　要：以德抗961(DK961)和泰山9818(TS9818)为试验材料,研究不同浓度外源硅对PEG胁迫下小麦幼苗的生长及抗氧化酶活性的影响。结果表明:与单纯PEG胁迫相比,外源硅浓度为1.0 mmol·L^(-1)时,DK961和TS9818的鲜重、干重及根系活力均最大,细胞膜透性最小,鲜重分别增加了32.85%和49.43%,干重分别增加了24.14%和50.00%,根系活力分别增加了18.97%和15.60%,细胞膜透性分别降低了12.53%和10.48%;外源硅浓度为0.5 mmol·L^(-1)时,DK961和TS9818的SOD、CAT活性均最大,分别增加了18.11%、19.71%和47.41%、44.51%;外源硅浓度分别为0.5 mmol·L^(-1)和0.1 mmol·L^(-1)时,DK961和TS9818的POD活性最大,MDA含量最低,POD活性分别增加了18.41%和13.02%,MDA含量分别降低了11.80%和12.31%。较低浓度的外源硅能有效促进PEG胁迫下小麦幼苗的生长,增加抗氧化酶活性,降低丙二醛含量。外源硅能够缓解干旱胁迫对小麦幼苗的危害,提高小麦抗旱能力。Using DK961 and TS9818 as experimental materials,the effects of different concentration of exogenous silicon on the growth of wheat seedlings and antioxidant enzyme activity under PEG stress were studied. Results indicated that: compared with only PEG stress,when the exogenous silicon concentration was 1. 0 mmol ·L^(-1),the fresh weight,the dry weight and the root activity of DK961 and TS9818 were the highest,increased by32. 85%,49. 43%,24. 14%,50. 00%,and 18. 97%,15. 60%,respectively,and the cell membrane permeability was the lowest,reduced by 12. 53% and 10. 48%,respectively. When exogenous silicon concentrations was0. 5 mmol·L^(-1),the SOD and CAT activities of DK961 and TS9818 were the largest,increased by 18. 11%,19.71%,and 47. 41%,19. 71%,respectively; when exogenous silicon concentrations were 0. 5 and 0. 1 mmol ·L^(-1),the POD activity of DK961 and TS9818 were the largest,increased by 18. 41% and 13. 02%,respectively,and the MDA content was the lowest,reduced by 11. 80% and 12. 31%,respectively. Lower concentration of exogenous silicon can promote the growth of wheat seedlings effectively under PEG stress,increasing the activity of antioxidant enzymes while reducing the content of MDA. Exogenous silicon can avoid the hazard of drought stress on wheat seedling.

关 键 词：外源硅 PEG胁迫 小麦 抗氧化酶 

分 类 号：S512.1[农业科学—作物学]