Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach  

作　　者：王辉 牛景彦 刘佳慧 杨洪帅 刘志刚 

机构地区：[1]Life Sciences College, Huaiyin Normal University [2]Polytechnic Department, Jiaozuo Teachers College [3]Fisheries College, Guangdong Ocean University

出　　处：《Chinese Journal of Oceanology and Limnology》2017年第3期537-545,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the Guangdong Province Science and Technology Project(No.2010B020201014);the Guangdong Province South China Sea Invertebrates Healthy Culture Engineering Technology Research Center(No.GCZX-A0909);the Guangdong Province Ocean and Fisheries Science and Technology Extension Project(No.20120980);the Guangdong Province Industry-University-Science Partnership Project(No.20110908);the Sci&Tech Plan of Huaiyin Normal University(No.WH0031)

摘　　要：The green alga Tetraselmis subcordiformis has been widely used as a quality live food for aquaculture species, and also has been studied as a model organism for the photo-biological production of hydrogen. We attempted to quantify the relationship between T. subcordiformis specific growth rate （SGR） and three important environmental factors （temperature, salinity, and pH） using the central composite design and response surface method under laboratory conditions. The results showed that the linear effects of temperature and salinity were significant （P〈0.05）, and they were equally important in impacting T. subcordiformis specific growth; the linear effect of pH was not significant （P〉0.05）; the interactive effect of temperature and pH was significant （P〈0.05）, whereas the temperature ~ salinity and salinity x pH interactions were not significant （P〉0.05）; all of the quadratic effects of the three factors were significant （P〈0.05）. A model equation for specific growth rate with the three factors was established, with the unadjusted and predictive R2 as high as 0.990 and 0.921, respectively, suggesting that the model was a very good fit and that it could be used to predict SGR. Through optimizing the reliable model, an optimal 3-factor combination of 25~C/35 of salinity/pH 7.9 was obtained, at which the maximum specific growth rate （0.65） was recorded, with a desirability value of 93.8%. These experimental results could serve as guidelines for increasing T. subcordiformis production efficiency.The green alga Tetraselmis subcordiformis has been widely used as a quality live food for aquaculture species, and also has been studied as a model organism for the photo-biological production of hydrogen. We attempted to quantify the relationship between T. subcordiformis specific growth rate(SGR) and three important environmental factors(temperature, salinity, and p H) using the central composite design and response surface method under laboratory conditions. The results showed that the linear effects of temperature and salinity were significant( P< 0.05), and they were equally important in impacting T. subcordiformis specific growth; the linear effect of p H was not significant( P >0.05); the interactive effect of temperature and p H was significant( P <0.05), whereas the temperature ′ salinity and salinity ′ p H interactions were not significant( P >0.05); all of the quadratic effects of the three factors were significant( P< 0.05). A model equation for specific growth rate with the three factors was established, with the unadjusted and predictive R^2 as high as 0.990 and 0.921, respectively, suggesting that the model was a very good fit and that it could be used to predict SGR. Through optimizing the reliable model, an optimal 3-factor combination of 25 ° C/35 of salinity/p H 7.9 was obtained, at which the maximum specific growth rate(0.65) was recorded, with a desirability value of 93.8%. These experimental results could serve as guidelines for increasing T. subcordiformis production efficiency.

关 键 词：Tetraselmis subcordiformis specific growth TEMPERATURE SALINITY PH growth model 

分 类 号：Q949.2[生物学—植物学]