Polymorphisms in the Myostatin-1 gene and their association with growth traits in Ancherythroculter nigrocauda  

作　　者：孙艳红 李清 王贵英 祝东梅 陈见 李佩 童金苟 

机构地区：[1]Wuhan Aquaculture Science Research Institute,Wuhan 430207,China [2]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China

出　　处：《Chinese Journal of Oceanology and Limnology》2017年第3期597-602,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Science Foundation for Young Scientists of China(No.31402296);the National Science Foundation for Post-Doctoral Scientists of China(No.2014M562077);the Wuhan Chenguang Project for Young Scholars(No.201372304010832)

摘　　要：Myostatin （MSTN） is a member of the transforming growth factor-β gene superfamily that negatively regulates skeletal muscle development and growth. In the present study, partial genomic fragments of Myostatin-1 （MSTN-1） in two commercial hatchery populations of Ancherythroculter nigrocauda, an economically important freshwater fish, were screened for single nucleotide polymorphisms （SNPs） and then genotyped by direct sequencing of PCR products. Five SNPs were identified in intron 1 and exon 2, including a non-synonymous mutation causing an amino acid change （Val to Ile） at position 180. Association analyses based on 300 individuals revealed that the g. 1129T〉C SNP locus was significantly associated with total length （TL）, body length （BL）, body height （BH） and body weight （BW） in 6- and 18-month-old populations, while the g. 1289G〉A locus was significantly associated with BH and BW in the 6-month-old population. Haplotype analyses revealed that fish with the genotype combinations TC/TC or TC/GA showed better growth performance. Our results suggest that g.l129T〉C and g.1289G〉A have positive effects on growth traits and may be candidate gene markers for marker-assisted selection in A. nigrocauda.Myostatin( MSTN) is a member of the transforming growth factor-β gene superfamily that negatively regulates skeletal muscle development and growth. In the present study, partial genomic fragments of Myostatin-1(MSTN-1) in two commercial hatchery populations of Ancheryth r oculter nigrocauda, an economically important freshwater fish, were screened for single nucleotide polymorphisms(SNPs) and then genotyped by direct sequencing of PCR products. Five SNPs were identified in intron 1 and exon 2, including a non-synonymous mutation causing an amino acid change(Val to Ile) at position 180. Association analyses based on 300 individuals revealed that the g.1129T>C SNP locus was significantly associated with total length(TL), body length(BL), body height(BH) and body weight(BW) in 6-and 18-month-old populations, while the g.1289G>A locus was significantly associated with BH and BW in the 6-month-old population. Haplotype analyses revealed that fish with the genotype combinations TC/TC or TC/GA showed better growth performance. Our results suggest that g.1129T>C and g.1289G>A have positive effects on growth traits and may be candidate gene markers for marker-assisted selection in A. nigrocauda.

关 键 词：Myostatin-1 （MSTN-1） single nucleotide polymorphisms （SNPs） Ancherythroculter nigrocauda growth traits association analysis 

分 类 号：S917.4[农业科学—水产科学]