3G-ELISA新方法在促肾上腺皮质素释素检测中的应用  

Application of a Novel 3G-ELISA Method in Corticotropin Releasing Hormone Detection

作　　者：李光宗[1] 陈锋[2] 刘英富[3] 张益[1] 郁硕 樊毫军[2] 侯世科[1] 

机构地区：[1]武警后勤学院附属医院救援医学研究所,天津300162 [2]武警后勤学院附属医院灾害应急救援医学全军重点实验室,天津300162 [3]武警后勤学院细胞生物与医学遗传学教研室,天津300309

出　　处：《生物技术通讯》2017年第2期137-140,共4页Letters in Biotechnology

摘　　要：目的:改进传统ELISA方法,提高其对痕量样本的检测灵敏度,降低检测限。方法:将一抗和二抗分别与纳米金和氧化石墨烯结合,应用于传统ELISA方法,通过纳米材料的信号放大作用,提高对痕量样本的检测灵敏度,降低检测限。结果:优化后的方法可对浓度为0.02μg/mL的促肾上腺皮质素释素进行检测,灵敏度提高了125倍。结论:改进后的方法性能稳定,重复性好,可用于生物样本中痕量被检测物的测定。Objective：To improve the sensitivity and lower detection limit of ELASA assay for the detection of trace samples by modifying primary antibody and secondary antibody. Methods：Gold nanoparticles and graphene oxide were introduced into the traditional ELASA assay to amplify the detection signal. Gold nanoparticles combined with primary antibody,and graphene oxide with second antibody. Results：The optimized method can detect corticotropin releasing hormone of 0.02 μg/mL,and the sensitivity was increased by 125-fold. Conclusion：The method is stable,repeatable and can be used to detect trace biological samples.

关 键 词：酶联免疫吸附测定 纳米金 氧化石墨烯 促肾上腺皮质素释素 

分 类 号：Q502[生物学—生物化学] R392.1[医药卫生—免疫学]