鹿茸动员大鼠骨髓内皮祖细胞促进缺血肢体血管新生的实验研究  

Experimental study of Pilos Antler in Mobilizing Endothelial Progenitor Cells for the Promotion of Angiogenesis in Ischemia Limbs of Rats

作　　者：贺怡然 李廷荃[2] 

机构地区：[1]山西中医药研究院,山西太原030012 [2]山西中医学院附属医院,山西太原030024

出　　处：《世界中西医结合杂志》2017年第3期349-352,共4页World Journal of Integrated Traditional and Western Medicine

基　　金：山西省卫生厅科研课题(201201096)

摘　　要：目的通过观察鹿茸对大鼠下肢缺血模型外周血骨髓来源内皮祖细胞(EPCs)数量及其增殖力、一氧化氮(NO)含量以及血管内皮生长因子(VEGF)的影响,探讨鹿茸促进缺血肢体血管新生的作用机制。方法采用结扎法建立大鼠下肢缺血模型,将90只雄性SD大鼠随机分为模型组,鹿茸高、中、低剂量组和复方丹参片组,空白组为同批次正常大鼠仅作皮肤切开缝合,每组各15只。各组大鼠每天灌胃1次,空白组和模型组给予等剂量蒸馏水,于术后第2天开始给药,连续给药2周,给药1周后分离、培养EPCs,然后收集贴壁细胞利用流式细胞仪来鉴定CD34/CD133表型,采用ELISA法检测VEGF的表达,使用CCK-8法检测细胞数量变化及其增殖力,并用硝酸还原酶法检测NO含量。结果鹿茸能够显著促进EPCs增殖,提高EPC的数量,增加外周血NO的含量,且提升VEGF的表达水平。结论鹿茸通过动员骨髓源EPCs进入外周血,促进局部的血管新生达到修复损伤的目的。Objective To explore the effect mechanism of pilos antler in promoting angiogenesis in ischemia limbs through observing its impacts on the count of endothelial progenitor cells（EPCs） in peripheral blood marrow in ischemic limbs of rat models, NO content and VEGF. Methods The isehemic model of hind limb was prepared by ligation in the rat. 90 male SD rats were randomized into a model group, a pilos antler high- dose, middle -dose and low- dose groups and a compound danshen tablets group. In a blank group, the normal rats of same batch were only prepared with incision and suture, 15 rats in each group. Lavage was done once every day in each group. The distilled water was used at same dose in the blank group and the model group. Medication was followed on the second day after surgery, continuously for 2 weeks. The rats were separated in 1 week after medication for EPCs cultivation. The adherent cells were collected. FCM was used to identify CD34/CD133 phenotype. ELISA was used to determine VEGF expression. CCK - 8 was used to determine the cell count and proliferation. The nitrale reduetase assay was used to determine NO content. Results Pilos antler promoted significantly EPC proliferation, increased EPC count and peripheral blood NO content and VEGF expression. Conclusion Pilos antler promotes local angiogenesis for the repair through mobilizing spinal cord EPCs flowing to peripheral blood.

关 键 词：大鼠下肢缺血模型 内皮祖细胞(EPCs) 一氧化氮(NO) 血管内皮生长因子(VEGF) 

分 类 号：R285.5[医药卫生—中药学] R543[医药卫生—中医学]