麦冬中具有生物活性的高异黄酮类化学成分研究  

Bioactive Homoisoflavonoids from Tuber of Ophiopogon japonicus

作　　者：姜醒[1] 王兴焱[1] 张春蕾[1] 吕红梅[1] 朱景鑫[1] 王加志[1] 赵敏[1] 苑光军[1] 

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《中国现代中药》2017年第4期491-495,共5页Modern Chinese Medicine

基　　金：黑龙江省自然基金项目(H201464)

摘　　要：目的:研究麦冬中高异黄酮类化合物及其生物活性。方法:利用多种色谱技术相结合的方法从麦冬中分离获得单体化合物,通过核磁共振方法确定化合物的结构。结果:从麦冬干燥块根中分离鉴定了10个化合物,分别为7-O-methyl-3'-hydroxy-3,9-dihydropunctatin(1),(3R)-3,7-dihydroxy-3-(4'-hydroxy-3'-methoxybenzyl)chroman-4-one(2),6-hydroxy-7-O-methyl-3,9-dihydropunctatin(3),7,4'-di-O-methyl-3'-hydroxy-3,9-dihydropunctatin(4),(3R)-3,7-dihydroxy-3-[furan-2'(5H)-one]chroman-4-one(5),(aS)-3,9,10-trihydroxy-7-hydro-6H-dibenz-[b,d]oxocin-7-one(6),(3R)-3-hydroxy-3-(4'-methoxybenzyl)-5,6,7-trimethoxychroman-4-one(7),(3R)-3-hydroxy-3-(4'-hydroxybenzyl)-5,6,7-trimethoxychroman-4-one(8),5-methoxy-6-methyl-7-hydroxy-8-aldehydo-3-(3',4'-methylenedioxybenzyl)chroman-4-one(9),5,8,4'-trimethoxy-6-methyl-7,2'-dihydroxy-3-benzylchroman-4-one(10)。体外细胞毒活性试验表明,化合物1、6、7具有抑制人肺癌细胞(A-549)增殖的活性,IC_(50)值分别为10.1、13.5、8.0μmol·L^(-1)。结论:化合物1~8为首次从麦冬中分离得到。Objective：To study the chemical constituents and bioactivities of the tuber of Ophiopogon japonicus.Methods：Single compounds were obtained by comprehensive chromatography technology.Structures were identified by nuclear magnetic resonance（NMR）technologyResults：Compounds isolated from the tuber were identified as 7-O-methyl-3′-hydroxy-3,9-dihydropunctatin（1）,（3R）-3,7-dihydroxy-3-（4′-hydroxy-3′-methoxybenzyl）chroman-4-one（2）,6-hydroxy-7-O-methyl-3,9-dihydropunctatin（3）,7,4′-di-O-methyl-3′-hydroxy-3,9-dihydropunctatin（4）,（3R）-3,7-dihydroxy-3-[furan-2′（5H）-one]chroman-4-one（5）,（aS）-3,9,10-trihydroxy-7-hydro-6H-dibenz-[b,d]oxocin-7-one（6）,（3R）-3-hydroxy-3-（4′-methoxybenzyl）-5,6,7-trimethoxychroman-4-one（7）,（3R）-3-hydroxy-3-（4′-hydroxybenzyl）-5,6,7-trimethoxychroman-4-one（8）,5-methoxy-6-methyl-7-hydroxy-8-aldehydo-3-（3′,4′-methylenedioxybenzyl）chroman-4-one（9）,5,8,4′-trimethoxy-6-methyl-7,2′-dihydroxy-3-benzylchroman-4-one（10）,respectivelyCompounds 1,6,and 7 exhibited cytotoxicity against A 549 with IC50 values of 10.1,13.5,8.0 μmol·L^-1,respectivelyConclusion：Compounds 1-8 were obtained from the plant for the first time.

关 键 词：麦冬 高异黄酮 细胞毒活性 人肺癌细胞 

分 类 号：R318.08[医药卫生—生物医学工程]