地鳖虫抗肿瘤成分提取方法的研究  

Study on Extraction of Antitumor Constituents from Eupolyphaga sinensis

作　　者：富尧[1] 张慧明[2] 许龙[1] 吕冬云[1] 

机构地区：[1]佳木斯大学生命科学学院,黑龙江佳木斯154002 [2]佳木斯大学基础医学院,黑龙江佳木斯154002

出　　处：《中国现代中药》2017年第4期557-559,571,共4页Modern Chinese Medicine

基　　金：佳木斯大学学研究生创新课题(LZR2014_002);佳木斯大学自然科学研究项目(JMSUJCMS2016-064)

摘　　要：目的:对地鳖虫的脂类提取物的最佳提取方法进行研究。方法:通过对索氏提取与超声法的对比,对超声时间、温度、原料浸泡时间、有机溶剂的选择进行实验,得出最佳的脂类提取条件。结果:本实验通过单因素分析,得出超声40 min时提取率为最高,达到17.23%,60 min时提取率反而下降为13.7%;超声温度在50℃时提取率最高,达到15.95%,随后在温度达到60℃时下降到14.02%,70℃时下降到10%以下;浸泡6 h时提取率最高,达到18.74%。而继续延长浸泡时间12、24、48 h,对脂类的提取量没有影响;有机溶剂无水乙醚提取率最高。结论:超声法应用无水乙醚浸泡6 h,超声温度50℃,超声时间40 min为地鳖虫脂类物质提取的最佳条件。Objective： To optimize the extraction of lipophile fraction of Eupolyphaga sinensis. Methods： Through the comparison of Soxhlet extraction and ultrasonic extraction, the extraction conditions for the lipophile fraction were optimized with ultrasonic time, temperature, soaking time of raw material, organic solvent as the factors. Results： In this study, single factor analysis, The extraction rate was 17. 23%, and the extraction rate decreased to 13.7% at 60 min; Ultrasonic temperature at 50 ℃ when the extraction rate of the highest, reaching 15.95%, then the temperature reached 60℃ to 14.02%. 70℃ down to 10% or less; The highest extraction rate was 18.74% when soaked for 6 h. While the prolonged soaking time 12h, 24h, 48h had no effect on the extraction of lipids; Organic solvent anhydrous ether extraction rate of the highest, Conclusion： The optimized condition was as follows： anhydrous ether soak 6 h, ultrasonic temperature 50, ultrasonic time 40 min.

关 键 词：地鳖虫 提取率 超声波 

分 类 号：R284.2[医药卫生—中药学]