呼吸道感染样本中IFITMs引物优化  

Optimization of IFITMs Primers in Respiratory Tract infection Samples

作　　者：左清利 鲁艳芹[1,2] 韩金祥[1,2] 

机构地区：[1]济南大学-山东省医学科学院医学与生命科学学院,山东济南250200 [2]山东省罕少见病重点实验室,山东省医药生物技术研究中心,山东省医学科学院,山东济南250062

出　　处：《罕少疾病杂志》2017年第2期20-21,40,F0002,共4页Journal of Rare and Uncommon Diseases

摘　　要：目的优化干扰素诱导的跨膜蛋白(Interferon induced transmenmbrane proteins,IFITMs)的五个亚型基因,即IFITM1、IFITM2、IFITM3、IFITM5和IFITM10。方法提取呼吸道感染样本中核酸,通过RT-q PCR筛选出具有特异性的引物且Ct值在[15,35]之间,再利用琼脂糖凝胶电泳对引物的特异性进行近一步的验证。结果 RT-q PCR结果显示IFITM1相关引物中IFITM1-3具有特异性;IFITM2相关引物中IFITM 2-2具有特异性;IFITM3相关引物IFITM3-2有较好特异性;IFITM5相关引物中IFITM5-4具有特异性;IFITM10相关引物中IFITM10-1、IFITM10-2、IFITM10-3具有特异性。具有特异性的所有引物的琼脂糖凝胶电泳条带单一明亮。结论 IFITM1-3优于该基因的其它引物;IFITM2-2优于IFITM2-1;IFITM3-2优于IFITM3-1;IFITM10-1、IFITM10-2和IFITM10-3均适用。Objective To optimize the five subtypes of interferon inducible transmembrane proteins, namely IFITM1, IFITM2, IFITM3, IFITM5 and IFITM10. Methods Extraction of nucleic acids from respiratory tract infection samples. Specific primers were screened by qRT-PCR and Ct value was between （15,35）, and the specificity of primers was verified by agarose gel electrophoresis. Results IFITM1-3 was specific in the IFITM1 primers. IFITM 2-2 was specific in the IFITM2 primers. IFITM3-2 was specific in the IFITM3 primers. IFITMS-6 was specific in the IFITM5 primers. IFITM 10-1, IFITM 10-2 and IFITM10-3 were specific in the IFITM 10 primers. Agarose gel electrophoresis bands of all primers with specificity were single bright, Concluaion IFITM1-3 was superior to other primers of IFITM1 primers, IFITM 2-2 was superior to other primers of IFITM2 primers. IFITM3-2 was superior to other primers of IFITM3 primers. IFITM5-4 was superior to other primers of IFITM5 primers. IFITM10-1, IFITM10-2 and IFITM10-3 were superior to other primers of IFITM10 primers.

关 键 词：呼吸道感染 引物 IFITMs 

分 类 号：R373.1[医药卫生—病原生物学]