长牡蛎消化系统胰岛素样蛋白的免疫组织化学定位  

Immunohistochemical Localization of Insulin-like Peptides in the Digestive System of Crassostrea gigas

作　　者：汲康 孙虎山[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264039

出　　处：《鲁东大学学报（自然科学版）》2017年第2期144-147,158,共5页Journal of Ludong University:Natural Science Edition

基　　金：山东省自然科学基金(ZR2012CM004)

摘　　要：长牡蛎(Crassostrea gigas)中存在胰岛素样蛋白,但胰岛素样蛋白在长牡蛎消化系统中是如何分布的尚不确定.为研究长牡蛎胰岛素样蛋白在消化系统中的分布情况,本研究采用ABC法免疫组织化学技术,对长牡蛎消化系统各组织器官中胰岛素样蛋白进行免疫组化定位研究.结果表明在长牡蛎胃、肠、直肠以及肝胰腺组织中均有阳性反应,在肝胰腺组织中阳性反应最强,腺小管中成局部集中分布,多集中分布于相对较大的外分泌细胞之间.推测长牡蛎肝胰腺有可能是长牡蛎胰岛素样蛋白的主要合成分泌器官.There is insulin-like peptides in Crassostrea gigas, but the distribution of insulin-like peptides in the digestive system is not known. In this research,immunohistochemical method of ABC was used to study the localization of insulin-like peptides in the tissues of the digestive system. The results showed that positive reaction appeared in the stomach, intestine, rectum and liver pancreatic tissues. The positive reaction was the strongest in the liver pancreas gland tubules, and it relatively concentrated in the glandular tubules, and it intensively distributed among relatively large exocrine cells. Therefore, we speculated that the liver pancreas of Crassostrea gigas might be the main organ of synthesis and secretion of insulin-like peptides.

关 键 词：长牡蛎 胰岛素样蛋白 免疫组化 

分 类 号：Q955[生物学—动物学]