使用两种培养基一种气体条件分离高原鼠兔肠道菌  

Enteric bacteria isolation from plateau pika with two media at the gas condition of 5% CO_2

作　　者：张桂[1] 杨晶[1] 金东[1] 卢珊[1] 徐建国[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《疾病监测》2017年第3期241-246,共6页Disease Surveillance

基　　金：国家自然科学基金(No.81290340;81290345);科技部生物安全重大研发项目(No.2016YFC1201903)~~

摘　　要：目的使用脑心浸液羊血培养基和KF链球菌羊血培养基对高原鼠兔肠道菌进行分离,以了解脑心浸液羊血培养基上所分离的细菌菌属的种类和丰度,同时用KF链球菌羊血培养基对高原鼠兔肠道菌进行选择性分离以了解高原鼠兔肠道中是否存在新种链球菌。方法使用脑心浸液羊血培养基和KF链球菌羊血培养基,在5%CO2气体条件下对高原鼠兔肠道菌进行培养和分离,使用16S r DNA全长序列分析等方法鉴定细菌。结果从20份高原鼠兔肠内容物标本中共分离607株细菌,可鉴定为19个属,31个种。其中用脑心浸液羊血培养基分离鉴定了495株细菌的19个属,用KF链球菌羊血培养基分离鉴定了112株细菌的3个属。分离鉴定的607株细菌,在属水平上,节杆菌属、肠球菌属、芽孢杆菌属为优势菌属,分别占31.47%、16.14%和15.98%;在种水平上,Arthrobacter luteolus、Enterococcus faecium为优势种。一些菌种为具有医学意义的细菌,如Yersinia enterocolitica、Staphylococcus haemolyticus、Staphylococcus epidermidis等。结论脑心浸液羊血培养基上所分离的菌属丰富,本次实验中尚未发现链球菌新种,分布广泛的青藏高原鼠兔肠道中携带多种致病菌和潜在致病菌。Objective To understand whether the new species of Streptococcus exists in the intestinal contents of plateau pika with brain heart infusion medim and KF medium. Methods With two media, i.e. heart infusion medium containing 5% defibrinated sheep blood and KF medium containing 5% defibrinated sheep blood, and one gas condition, i. e. 5% CO2, microorganism was isolated from intestinal contents of plateau pika. The identification was done by using full length of 16S rDNA. Results A total of 607 strains were isolated, which were identified into 19 genera and 31 species. And 495 strains in 19 genera were identified by using the medium of brain heart infusion containing 5% defibrinated sheep blood, and 112 strains in 3 genera were identified by using KF medium containing 5% defibrinated sheep blood. At the level of genus, Arthrobacter, Enterococcus, Bacillus were predominant, accounting for 31.47%, 16. 14% and 15.98% respectively. The most abundant species were Arthrobacter luteolus, Enterococcus faecium. Some identified species were with medical significance, including Yersinia enterocolitica,Staphylococcus haemolyticus, Staphylococcus epidermidis. Conclusion Bacteria in abundant genera were isolated from the intestinal contents of plateau pika with brain heart infusion medium with 5% defibrinated sheep blood,and the new species of Streptococcus was not found in this experiment. The plateau pika in Qinghai-Tibet area carry some bacterial pathogens.

关 键 词：高原鼠兔 肠内容物 多培养方式 菌株分离 

分 类 号：R446.5[医药卫生—诊断学]