应用细胞色素C氧化酶亚基Ⅰ和16S rRNA基因鉴定浙江口岸鼠类及其序列特征分析  

Identification of rodent species in Zhejiang port using cytochrome C oxidase subunitⅠ and 16S rRNA gene and analysis of sequence characteristics

作　　者：齐润姿 王董[2] 普丽娅 赵欣[2] 高雪萌 马翔 余斌 张晓龙[2] 

机构地区：[1]浙江出入境检验检疫局,杭州310016 [2]中国检验检疫科学研究院,北京100176 [3]温州出入境检验检疫局,浙江温州325027

出　　处：《中国媒介生物学及控制杂志》2017年第2期104-107,116,共5页Chinese Journal of Vector Biology and Control

基　　金：国家质检总局科技计划项目(2015IK303)~~

摘　　要：目的鉴定浙江口岸鼠类,并对其序列特征进行分析。方法于2015年4-10月在浙江口岸捕获鼠类,取其肝脏,对捕获鼠类的线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)、16S rRNA序列片段进行扩增,鉴定鼠种并构建系统发育树。结果浙江口岸捕获的鼠类为褐家鼠和小家鼠。COⅠ基因变异率大于16S rRNA基因,均以转换为主,且COⅠ基因以同义突变为主。结论 COⅠ和16S rRNA基因均可作为鼠类鉴定的分子标记。Objective To idemify rodent species in Zhejiang port and analyze the sequence characteristics. Methods The livers were obtained from rodents captured during April to October in 2015. The cytochrome C oxidase subunit Ⅰ （CO Ⅰ ） and 16S rRNA gene sequences of rodents were amplified. The species were confirmed and the phylogenetic trees were constructed. Results There were two species, Rattus norvegicus and Mus musculus in Zhejiang port. The mutation rate of CO I gene was greater than that of 16S rRNA gene. Most of the mutation was transition and synonymous mutation. Conclusion Both CO I gene and 16S rRNA gene, as molecular markers, could be applied to identify rodent species.

关 键 词：鼠类 系统发育树 鉴定 

分 类 号：S443[农业科学—农业昆虫与害虫防治]