北京市朝阳区首次从蚊虫标本中检出辽宁病毒  

First report of Liaoning virus from mosquitoes in Chaoyang district,Beijing

作　　者：唐承军[1] 葛军旗[1] 张洪江[1] 付士红[2] 李元元[2] 徐潮[3] 王会平[1] 张政[1] 

机构地区：[1]北京市朝阳区疾病预防控制中心消毒科,北京100021 [2]中国疾病预防控制中心病毒病预防控制所,北京102206 [3]兰州大学,兰州730000

出　　处：《中国媒介生物学及控制杂志》2017年第2期113-116,共4页Chinese Journal of Vector Biology and Control

基　　金：北京市优秀人才培养资助项目(2014000052580G290);北京市朝阳区疾病预防控制中心自选项目(Cycdc-2015-271103)~~

摘　　要：目的了解北京市朝阳区蚊虫携带辽宁病毒(LNV)情况。方法按照《病媒生物密度监测方法蚊虫》(GB/T 23797-2009)中的方法,于2014-2015年采用CO2诱蚊灯采集蚊虫,利用反转录聚合酶链式反应检测LNV核酸,采用Mega 6.0软件进行进化分析。结果 2014-2015年共采集蚊虫9 811只,其中淡色库蚊为优势种,占捕获总数的91.41%(8 968/9 811)。标本编号BJCY14007的淡色库蚊核酸经检测为LNV阳性。基于LNV第10节段的分子特征显示,LNV分支为两个进化群血清Ⅰ型和血清Ⅱ型,BJCY14007与NE97-12较为接近,属血清Ⅰ型。结论北京市首次报道从蚊虫中检出LNV。因LNV有潜在的致病性,应注意其分布及变化情况,且有必要在人群及宿主动物中开展血清学调查。Objective To investigate Liaoning virus （LNV） in mosquitoes in Chaoyang district, Beijing. Methods According to the Surveillance for Vector Density-Mosquito （GB/T 23797-2009）, mosquitoes were collected by CO2 traps in 2014 and 2015. Reverse transcription-polymerase chain reaction was used to detect LNV. Molecular characteristics were carried out by Mega 6.0. Results A total of 9 811 mosquitoes were collected and Culex pipiens pallens （8 968/9 811, 91.41%） was the main species. The sample BJCY14007 was positive for LNV. LNV has two branches （serotype I and serotype II） based on the molecular characteristics of the segment 10. BJCY14007 was more related to NE97-12 （representative strain, serotype I ）. Conclusion The LNV was firstly detected in Beijing. It deserves further investigation LNV prevalence in mosquito, human population and other hosts for risk assessment.

关 键 词：虫媒病毒 辽宁病毒 分子特征 北京市 

分 类 号：R373.3[医药卫生—病原生物学] R384.1[医药卫生—基础医学]