流动注射-抑制化学发光法测定岩白菜素  

Determination of Bergenin by Flow Injection Analysis Coupled with Inhibited Chemiluminescence System

作　　者：张韫[1] 高苏亚[1] 扈本荃[1] 

机构地区：[1]西安医学院药学院,陕西西安710021

出　　处：《分析科学学报》2017年第2期227-231,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21175106);陕西省大学生创新创业训练计划(No.2312);西安医学院青年科研基金(No.2015QN04);西安医学院大学生科研训练基金(No.2015DXS1-24)

摘　　要：本文基于岩白菜素对鲁米诺-牛血清白蛋白体系化学发光信号显著的抑制作用,建立了快速灵敏测定岩白菜素的流动注射-化学发光分析新方法。实验发现,化学发光强度的降低值与岩白菜素质量浓度对数值呈良好的线性关系,方法的线性范围为3.0~5.0×10~5 pg/mL,检出限(3σ)为1.0pg/mL。当溶液流速为2.0mL/min时,完成一次分析过程仅需30s,采样频率120/h。本法用于片剂、人血清和尿液中岩白菜素的含量测定,回收率为98.1%~102.7%,相对标准偏差小于2.0%(n=7)。同时对化学发光反应机理进行了探讨。A rapid and sensitive flow injection-chemiluminescence（FI-CL）method for the determination of bergenin was established,based on the inhibition effect of bergenin on luminol-bull serum albumin system.It was found that the decrement of CL intensity was linearly proportional to the logarithm of bergenin concentration ranging from 3.0 to 5.0×10~5 pg/mL,with the limit of detection of 1.0pg/mL（3σ）.At the flow rate of 2.0mL/min,a typical analytical procedure including sampling and washing was finished within 30 s,which offered sample efficiency of 120/h.The proposed method has been applied to the determination of bergenin in tablets,human serum and urine samples with the recoveries of 98.1%-102.7%,and the RSDs of less than 2.0%（n=7）.The possible mechanism of the CL reaction was also proposed.

关 键 词：岩白菜素 化学发光 流动注射 鲁米诺 牛血清白蛋白 

分 类 号：O657.39[理学—分析化学]