分子印迹Mn掺杂ZnS量子点磷光法检测烟酸转化液中的6-羟基烟酸  

Detection of 6-Hydroxynicotinic Acid in Nicotinic Acid Conversion Fluid Using Molecularly Imprinted Polymer Capped Mn-doped ZnS Phosphorescent Quantum Dots

作　　者：张文刚[1] 雒雪丽 韩雍[1] 李忠宏[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《分析测试学报》2017年第4期478-483,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(31371813);国家科技部(863计划)项目资助(2013FY113400)

摘　　要：以6-羟基烟酸(6-HNA)为模板,采用分子印迹溶胶-凝胶法合成了具有良好光学性质的印迹Mn掺杂Zn S量子点磷光传感器(MIP-QDs)。红外光谱(FT-IR)和扫描电镜(SEM)测试表明,印迹聚合物成功接枝在纳米传感器表面。吸附试验显示,MIP-QDs对6-HNA的亲和性与选择性显著高于其结构类似物阔马酸和烟酸,具备作为检测探针的潜力。在最优条件下,基于6-HNA可选择性猝灭MIP-QDs磷光而建立了灵敏、简便的检测微量6-HNA的光学新方法。当6-HNA浓度在3.4~67.0μmol/L范围内,MIP-QDs的磷光猝灭率P_0/P随浓度变化的关系符合Stern-Volmer方程(r^2=0.996 5),检出限为1.03μmol/L。实际样品测定显示,6-HNA的回收率为91.0%~103.9%,相对标准偏差不超过4.5%,检测结果与HPLC法相一致(P>0.05),方法具有良好的准确性和可行性。An optical nanosensor based Mn doped ZnS quantum dots（QDs）was synthesized using 6-hydroxynictinic acid（6-HNA） as template via surface molecular imprinting sol-gel method.Fourier transform infrared spectroscopic（FT-IR） spectra and scanning electron microscopic（SEM） images showed that the imprinted polymer was successfully grafted onto MIP-QDs.Adsorption tests revealed that the affinity and selectivity of MIP-QDs for 6-HNA were significantly higher than its analogues nicotinic acid and coumalic acid.Under the optimized conditions,a phosphorescent sensor for detecting 6-HNA was constructed.The linear range for detection of 6-HNA was in the range of 3.4-67.0 μmol/L with a detection limit of 1.03 μmol/L and a correlation coefficient（r2） of 0.996 5.Recoveries of 91.0%-103.9% and relative standard deviations less than 4.5% were achieved in real samples,which were in good agreement with that of the high performance liquid chromatographic（HPLC） method（P〉0.05）.The proposed method had good veracity and feasibility.

关 键 词：量子点 分子印迹 磷光检测 6-羟基烟酸(6-HNA) 烟酸 

分 类 号：O657.3[理学—分析化学] TQ216[理学—化学]