分光光度法测定聚乙二醇-壳聚糖的接枝率  

Determination of the Grafting Ratio of Chitosan-graft-polyethylene Glycol by Spectrophotometry Method

作　　者：朱志敏[1] 邓湘萍 熊淑娟[1] 王哲[1] 熊润德 邹柳[1] 刘娟[1] 唐国涛[1] 

机构地区：[1]南华大学药物药理研究所,湖南省分子靶标新药研究协同创新中心,湖南衡阳421001

出　　处：《广东化工》2017年第7期227-228,224,共3页Guangdong Chemical Industry

摘　　要：目的用亲水性的聚乙二醇对壳聚糖进行改性,探索分光光度法对聚乙二醇-壳聚糖聚合物中聚乙二醇接枝率的检测方法。方法通过化学方法合成亲水性mPEGCOONSu活化物,将其连接壳聚糖的氨基侧链上,得到改性了的聚乙二醇-壳聚糖接枝产物;用直接和间接两种分光光度法检测聚乙二醇-壳聚糖接枝产物的接枝率,并用核磁共振方法辅助证明。结果 ~1H-NMR确认了聚乙二醇-壳聚糖接枝的合成,测得接枝率为15.69±2.38%,直接间接两种方法测定接枝率分别为17.25±2.56%和16.34±1.98%。结论采用分光光度法测定聚乙二醇-壳聚糖的接枝率是一种简便、可行、有效的测定方法。Objective： Chitosan was modified with hydrophilic polyethylene glycol, and exploring the method of determination of the grafting ratio of chitosan-graft-poiyethylene glycol by Spectrophotometry. Method： Chitosan-grafi-polyethylene glycol was obtained through grafting hydrophilic mPEG spacer to the amino group of chitosan. The grafting ratio of chitosan-graft-polyethylene glycol was determined by direct and indirect spectrophotometry, and proved by nuclear magnetic resonance method. Results： Through t H-NMR experiment, the grafting ratio of chitosan-graft-polyethylene glycol was 15.69~2.38 %. The grafting ratio of chilosan-grafl-polyethylene glycol was 7.25~2.56 % and 16.34~1.98 % by direct and indirect spectrophotometry method. Conclusion： It is a simple, feasible and eft＇cctive method to determine the grafting ratio of chitosan-graft-polyethylene glycol by spectrophotometry.

关 键 词：壳聚糖 聚乙二醇 接枝率 分光光度法 

分 类 号：TQ[化学工程]