大菱鲆脂联素受体基因的克隆及饲料糖脂比对其表达的影响  

MOLECULAR CLONING OF ADIPONECTIN RECEPTORS IN TURBOT SCOPHTHALMUS MAXIMUS AND ITS EXPRESSION RESPONSE TO DIETARY RATIOS OF CARBOHYDRATE TO LIPID

作　　者：韩冬冬[1] 苗淑彦[1,2] 聂琴[1] 苗惠君[1] 魏泽宏 张文兵[1] 麦康森[1] 

机构地区：[1]中国海洋大学水产学院,农业部水产动物营养与饲料重点实验室,海水养殖教育部重点实验室,青岛266003 [2]扬州大学动物科学与技术学院,扬州225009

出　　处：《水生生物学报》2017年第3期565-572,共8页Acta Hydrobiologica Sinica

基　　金：国家973计划项目(2014CB138600)资助~~

摘　　要：脂联素作为一种重要的细胞因子在哺乳动物中起到调节糖脂代谢的重要作用,为探究脂联素在大菱鲆糖脂代谢中是否具有同样的调节作用,研究利用分子克隆和RACE技术克隆到了大菱鲆脂联素Adipo R1受体和Adipo R2受体的基因全长序列。其中Adipo R1受体的核苷酸序列全长为1125 bp,共编码375个氨基酸,氨基酸序列与其他脊椎的同源性都很高。Adipo R2受体的核苷酸序列全长为1940 bp,共编码380个氨基酸,氨基酸序列与其他脊椎动物同源性也都很高。蛋白跨膜结构域预测得出大菱鲆Adipo R1和Adipo R2都具有7个明显的跨膜区,最优拓扑模型为N端在细胞内侧模型,与其他脊椎动物相同。配制不同糖脂比(1鲶6,1鲶2,2鲶1和14鲶1)的饲料养殖大菱鲆9周,利用实时荧光定量PCR技术检测大菱鲆肌肉和肝脏中脂联素受体基因的表达量。结果显示,在饲料糖脂比为1鲶2的处理组中,大菱鲆肌肉中Adipo R1的表达量显著低于糖脂比为1鲶6的处理组(P<0.05),但与其他2组无显著差异。饲料糖脂比的变化对肌肉中Adipo R2和肝脏中这2种受体的表达量均没有产生显著影响(P>0.05)。由此可见,饲料糖脂比的变化可能通过改变脂联素受体的表达量,从而间接影响大菱鲆体内脂联素的作用。Adipo R1和Adipo R2对饲料糖脂比变化的反应不同步,而且肌肉和肝脏中脂联素受体对饲料糖脂比变化的反应也存在不同。适量提高饲料糖脂比可以显著下调肌肉中Adipo R1的表达。The present study cloned the full-length cDNA sequences of adiponectin receptors （AdipoR1, AdipoR2） inturbot. The cDNA sequence of AdipoR1 was 1125 bp, encoding 375 amino acids. The deduced amino acid sequence ofAdipoR1 was conserved with the vertebrate’s AdipoR1, such as human （80%） and tilapia （97%）. The cDNA sequenceof AdipoR2 was 1940 bp, encoding 380 amino acids. The amino acid sequence of AdipoR2 was 78% identical to human and 92% to tilapia. Like invertebrates, both AdipoR1 and AdipoR2 had 7 transmembrane domains, and the N-terminal of the optimal topological structure was inside cells. Turbot adiponectin receptors were widely expressed in alldetected tissues. The highest mRNA level of AdipoR1 was found in gill and kidney. The high level of AdipoR2 wasfound in gill, stomach, intestine, kidney and pyloric caeca. A 9-week feeding trial using diets with different ratios ofcarbohydrate to lipid （1：6, 1：2, 2：1 and 14：1） indicated that 1：2 dietary carbohydrate-to-lipid ratio but not other two diets significantly decreased the expression of AdipoR1 （P〈0.05） in turbot muscle. All 3 diets had no significant effect onthe AdipoR2 expression in muscle and the expressions of AdipoR1 and AdipoR2 in liver （P〉0.05）. These results suggest that the dietary carbohydrate-to-lipid ratios may influence the function of adiponectin through changing the adipon-ectin receptors expression in muscle.

关 键 词：大菱鲆 脂联素 脂联素受体 糖脂比 基因表达 

分 类 号：S965.3[农业科学—水产养殖]